{"product_id":"eif1ax-antibody-eif1a-x-isoform-bha17135676","title":"EIF1AX Antibody \/ eIF1A X isoform","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eEIF1AX Antibody \/ eIF1A X isoform is a anti-EIF1AX Rabbit antibody Recombinant Rabbit Monoclonal clone 29E78 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e EIF1AX\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 29E78, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eEIF1AX antibody detects eukaryotic translation initiation factor 1A X linked isoform, encoded by the EIF1AX gene. Common names include eIF1A X isoform, translation initiation factor SUI1 homolog, and X linked eukaryotic translation initiation factor 1A. EIF1AX is a small but critical initiation factor that promotes scanning of mRNA and start codon recognition during translation initiation. It interacts with the 40S ribosomal subunit and other initiation factors to assemble the translation preinitiation complex, ensuring accurate start site selection and efficient protein synthesis.\u003cbr\u003e\u003cbr\u003eEIF1AX antibody is widely applied in molecular biology, cancer research, and developmental studies. Translation initiation is a highly regulated step in gene expression, and EIF1AX ensures fidelity in AUG codon selection. Mutations in EIF1AX have been identified in uveal melanoma, thyroid cancer, and endometrial carcinoma, where they cooperate with other oncogenic events. By detecting EIF1AX, researchers can study how altered initiation contributes to tumor biology and translational control.\u003cbr\u003e\u003cbr\u003eApplications for EIF1AX antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects EIF1AX expression in cell lysates, immunohistochemistry maps its expression in tumors and tissues, and immunofluorescence highlights nuclear and cytoplasmic localization. These assays provide strong tools to evaluate translation initiation factor biology in both normal and disease contexts.\u003cbr\u003e\u003cbr\u003eEIF1AX mutations often cluster at the N terminus, affecting interactions with other initiation factors and altering start codon selection. Dysregulation of initiation leads to abnormal gene expression patterns, contributing to cell proliferation and tumorigenesis. By applying EIF1AX antibody, scientists can study these processes in detail and evaluate EIF1AX as a potential biomarker or therapeutic target.\u003cbr\u003e\u003cbr\u003eEIF1AX also functions in normal development, where accurate translation initiation is critical for embryogenesis and tissue growth. Its conservation across species highlights its fundamental role in biology. The antibody therefore supports research from basic ribosome biology to applied cancer translational studies.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=EIF1AX - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=EIF1AX - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=EIF1AX - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/EIF1AX - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=EIF1AX+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047299899757,"sku":"FY12773","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_3e246dbb-6220-4024-83e4-aeaa84828039.jpg?v=1772019340","url":"https:\/\/www.ebiohippo.com\/products\/eif1ax-antibody-eif1a-x-isoform-bha17135676","provider":"BioHippo","version":"1.0","type":"link"}