{"product_id":"eif1ax-antibody-eif1a-x-isoform-bha17135963","title":"EIF1AX Antibody \/ eIF1A X isoform","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eEIF1AX Antibody \/ eIF1A X isoform is a anti-EIF1AX Rabbit antibody Recombinant Rabbit Monoclonal clone 29E67 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e EIF1AX\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 29E67, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF, IP\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eEIF1AX antibody detects Eukaryotic translation initiation factor 1A X linked, encoded by the EIF1AX gene. This protein is a translation initiation factor that plays an essential role in assembling the 43S preinitiation complex, stabilizing Met tRNAi binding to the 40S ribosomal subunit, and scanning mRNAs for start codons. EIF1AX antibody provides researchers with a precise reagent for exploring how initiation factors regulate protein synthesis and contribute to disease.\u003cbr\u003e\u003cbr\u003eEukaryotic translation initiation factor 1A X linked is a member of the EIF1A family, which includes autosomal EIF1A and the X chromosome encoded isoform. Although both proteins share significant homology and overlapping function, EIF1AX has unique tissue expression and regulatory features. Research using EIF1AX antibody has demonstrated that depletion of this factor disrupts translation initiation, leading to defective ribosome loading and reduced protein synthesis. This highlights its central role in the earliest step of gene expression control.\u003cbr\u003e\u003cbr\u003eMutations in EIF1AX have been identified in several cancers, including uveal melanoma, thyroid carcinoma, and ovarian cancer. These mutations cluster in the N terminal tail of the protein and are thought to alter ribosomal scanning or initiation fidelity. Studies with EIF1AX antibody have shown that mutated EIF1AX expression alters global translation profiles, potentially driving oncogenesis by enhancing the synthesis of pro survival or proliferative proteins. This makes EIF1AX both a cancer biomarker and a potential therapeutic target.\u003cbr\u003e\u003cbr\u003eBeyond cancer, EIF1AX is essential for development and cellular homeostasis. Research using EIF1AX antibody has revealed that knockdown in model organisms causes embryonic lethality due to failure of translation initiation. Its expression is enriched in proliferative tissues, consistent with high protein synthesis demands. Because translation initiation is tightly linked to growth signals such as mTOR and MAPK pathways, EIF1AX occupies a central position in connecting signaling to protein production.\u003cbr\u003e\u003cbr\u003eEIF1AX antibody is used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein expression across proliferative cell lines, immunohistochemistry localizes EIF1AX in tissue sections, and immunofluorescence highlights its presence in the cytoplasm and nucleus. These techniques allow researchers to assess expression changes, localization, and associations with ribosomal components. Functional studies with EIF1AX antibody include co immunoprecipitation, revealing its interactions with initiation complex proteins and ribosomal subunits.\u003cbr\u003e\u003cbr\u003eBy supplying validated EIF1AX antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=EIF1AX - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=EIF1AX - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=EIF1AX - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/EIF1AX - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=EIF1AX+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047310745965,"sku":"FY13061","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_6f034751-db31-4c02-ad10-d74b0eddbbc6.jpg?v=1772019385","url":"https:\/\/www.ebiohippo.com\/products\/eif1ax-antibody-eif1a-x-isoform-bha17135963","provider":"BioHippo","version":"1.0","type":"link"}