{"product_id":"eif2a-antibody-eukaryotic-translation-initiation-factor-2a-bha17135970","title":"EIF2A Antibody \/ Eukaryotic translation initiation factor 2A","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eEIF2A Antibody \/ Eukaryotic translation initiation factor 2A is a anti-EIF2A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e EIF2A\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eEIF2A antibody detects Eukaryotic translation initiation factor 2A, a noncanonical initiation factor that facilitates translation initiation at non-AUG codons and reinitiation after upstream open reading frames. The UniProt recommended name is Eukaryotic translation initiation factor 2A (EIF2A). This factor functions independently of EIF2 and promotes initiation under stress or viral infection conditions when canonical EIF2-dependent translation is inhibited.\u003cbr\u003e\u003cbr\u003eFunctionally, EIF2A antibody recognizes a 585-amino-acid cytoplasmic protein that mediates the recruitment of initiator Met-tRNA to the ribosome in an EIF2-independent manner. EIF2A interacts with EIF3, ribosomal subunits, and tRNA to enable translation initiation, particularly for internal ribosome entry site (IRES)-containing mRNAs or stress-induced transcripts. Its activity contrasts with EIF2S1 (EIF2-alpha), which forms the classic EIF2 complex with GTP and initiator tRNA.\u003cbr\u003e\u003cbr\u003eThe EIF2A gene is located on chromosome 3q26.2 and is ubiquitously expressed, with high levels in pancreas, liver, and testis. During cellular stress, EIF2A supports translation of mRNAs involved in survival and recovery, including those that escape EIF2-alpha phosphorylation-mediated repression. It also contributes to viral mRNA translation, highlighting its role in host-pathogen interactions.\u003cbr\u003e\u003cbr\u003ePathologically, altered EIF2A function has been linked to metabolic disorders, neurodegenerative diseases, and cancer. Its role in selective translation during stress responses connects it to the unfolded protein response and integrated stress response pathways. Research with EIF2A antibody enables detection of translational shifts and analysis of noncanonical initiation mechanisms.\u003cbr\u003e\u003cbr\u003eEIF2A antibody is suitable for western blot, immunocytochemistry, and immunoprecipitation to detect EIF2A in cultured cells and tissues. It is commonly used in studies of translational control, stress granule dynamics, and viral infection models. \u003cbr\u003e\u003cbr\u003eStructurally, EIF2A is composed of N-terminal domains that interact with tRNA and ribosomal proteins, and C-terminal extensions involved in EIF3 binding. It differs mechanistically from EIF2S1 in GTP usage and codon selection. This antibody helps delineate EIF2A's contribution to alternative initiation and translational adaptation under stress conditions.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=EIF2A - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=EIF2A - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=EIF2A - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/EIF2A - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=EIF2A+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047311040877,"sku":"FY13068","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2ce17318-92e5-4d44-b38e-b2e5a23a51b0.jpg?v=1782237082","url":"https:\/\/www.ebiohippo.com\/products\/eif2a-antibody-eukaryotic-translation-initiation-factor-2a-bha17135970","provider":"BioHippo","version":"1.0","type":"link"}