{"product_id":"eif4a3-antibody-eukaryotic-initiation-factor-4a-iii-bha17136256","title":"EIF4A3 Antibody \/ Eukaryotic initiation factor 4A-III","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eEIF4A3 Antibody \/ Eukaryotic initiation factor 4A-III is a anti-EIF4A3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e EIF4A3\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eEIF4A3 antibody detects Eukaryotic initiation factor 4A-III, an RNA helicase encoded by the EIF4A3 gene located on chromosome 17q25.3. EIF4A3 is a nuclear and cytoplasmic ATP-dependent RNA helicase belonging to the DEAD-box family and serves as a core component of the exon junction complex (EJC). The protein is essential for mRNA splicing, export, surveillance, and translation regulation. EIF4A3 is ubiquitously expressed but shows particularly high levels in brain, testis, and proliferating tissues where mRNA metabolism is active.\u003cbr\u003e\u003cbr\u003eEIF4A3 functions as an mRNA quality control factor by binding to spliced mRNAs upstream of exon-exon junctions as part of the EJC, together with MAGOH, RBM8A (Y14), and MLN51. This complex regulates nonsense-mediated mRNA decay and translation efficiency, ensuring that defective transcripts are degraded. EIF4A3 also acts independently as an RNA helicase that unwinds RNA secondary structures to facilitate ribosome scanning during translation initiation. Co-localization studies show EIF4A3 concentrated in nuclear speckles and cytoplasmic ribonucleoprotein granules, reflecting its dual role in RNA processing and translation.\u003cbr\u003e\u003cbr\u003eStructurally, EIF4A3 contains two RecA-like domains forming an ATP-binding cleft and conserved motifs such as the DEAD (Asp-Glu-Ala-Asp) box essential for helicase activity. It belongs to the DEAD-box helicase subfamily of eukaryotic initiation factors, which also includes EIF4A1 and EIF4A2. Unlike these cytoplasmic isoforms, EIF4A3 primarily functions in the nucleus as part of the EJC, anchoring the complex to mRNA. Known interacting partners include MAGOH, Y14, UPF1, and CASC3.\u003cbr\u003e\u003cbr\u003eFunctionally, EIF4A3 is indispensable for mRNA stability, nonsense-mediated decay, and post-transcriptional gene regulation. It coordinates RNA splicing and surveillance with translation, maintaining proteome integrity. In neurons, EIF4A3 regulates synaptic mRNA localization and local protein synthesis critical for plasticity. During embryonic development, EIF4A3 contributes to neural differentiation and morphogenesis by regulating gene expression at the RNA level.\u003cbr\u003e\u003cbr\u003eMutations or depletion of EIF4A3 disrupts RNA surveillance, leading to aberrant transcript accumulation and developmental defects. Loss-of-function variants cause Richieri-Costa-Pereira syndrome, characterized by craniofacial and limb malformations. Dysregulation of EIF4A3 expression has also been associated with tumor progression, as enhanced RNA surveillance supports oncogenic growth. Pathway associations include mRNA splicing, nonsense-mediated decay, and translational control.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using EIF4A3 antibody demonstrates nuclear and cytoplasmic localization in neurons, epithelial cells, and germ cells. The EIF4A3 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=EIF4A3 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=EIF4A3 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=EIF4A3 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/EIF4A3 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=EIF4A3+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323001197,"sku":"FY13354","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_837cfd0f-be4e-435c-824f-053367484f1f.jpg?v=1782237104","url":"https:\/\/www.ebiohippo.com\/products\/eif4a3-antibody-eukaryotic-initiation-factor-4a-iii-bha17136256","provider":"BioHippo","version":"1.0","type":"link"}