{"product_id":"elob-antibody-elongin-b-bha17135508","title":"ELOB Antibody \/ Elongin B","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eELOB Antibody \/ Elongin B is a anti-ELOB Rabbit antibody Recombinant Rabbit Monoclonal clone 30E87 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ELOB\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30E87, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eELOB antibody detects elongin-B, a transcriptional regulatory protein encoded by the ELOB gene. Elongin-B is a subunit of the elongin complex, which also includes elongin-C and elongin-A. This complex functions as a transcription elongation factor that enhances the rate of RNA polymerase II mediated transcription by suppressing transient pausing. In addition, elongin-B and elongin-C form a stable heterodimer that associates with cullin-RING ubiquitin ligases, linking ELOB to protein degradation pathways.\u003cbr\u003e\u003cbr\u003eELOB antibody is widely applied in molecular biology, transcription, and ubiquitin proteasome system research. Through its participation in the elongin complex, ELOB regulates transcriptional control of genes involved in growth, survival, and stress responses. In ubiquitination, ELOB contributes to assembly of the von Hippel-Lindau tumor suppressor complex, which targets hypoxia-inducible factors for degradation. By detecting elongin-B, researchers can investigate how ELOB coordinates transcription and protein turnover.\u003cbr\u003e\u003cbr\u003e Western blot assays detect ELOB protein in nuclear extracts, while immunohistochemistry maps expression in diverse tissues. Immunofluorescence reveals localization in nuclei and cytoplasmic ubiquitin ligase complexes. These applications enable comprehensive study of elongin-B across cellular pathways.\u003cbr\u003e\u003cbr\u003eAltered ELOB function has been associated with cancer, where deregulated ubiquitination and transcription drive tumor progression. Mutations affecting the VHL-elongin-C-B complex contribute to renal cell carcinoma by stabilizing hypoxia-inducible factors. By applying ELOB antibody, scientists can explore its role in tumor suppression, protein degradation, and transcriptional regulation.\u003cbr\u003e\u003cbr\u003eBeyond oncology, elongin-B plays roles in stress responses and cellular adaptation to hypoxia. Its broad functions reflect its integration of gene expression and protein stability mechanisms. This versatility makes ELOB antibody valuable for both basic and disease related research.\u003cbr\u003e\u003cbr\u003eELOB antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ELOB - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ELOB - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ELOB - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ELOB - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ELOB+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047295410541,"sku":"FY12605","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5c96a710-edbf-4bac-84f7-a496a6ea90f0.jpg?v=1772019314","url":"https:\/\/www.ebiohippo.com\/products\/elob-antibody-elongin-b-bha17135508","provider":"BioHippo","version":"1.0","type":"link"}