{"product_id":"elob-antibody-elongin-b-bha17135592","title":"ELOB Antibody \/ Elongin B","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eELOB Antibody \/ Elongin B is a anti-ELOB Rabbit antibody Recombinant Rabbit Monoclonal clone 30E89 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ELOB\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 30E89, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eELOB antibody detects elongin B, a protein encoded by the ELOB gene. Elongin B is part of the elongin complex, which also includes elongin C and elongin A. This complex functions as a transcription elongation factor by increasing the rate of RNA polymerase II transcription through transiently pausing sites. Elongin B also acts as a subunit of ubiquitin ligase complexes, linking it to protein degradation pathways.\u003cbr\u003e\u003cbr\u003eELOB antibody is widely applied in transcription research, ubiquitin signaling, and cancer biology. As part of the elongin complex, elongin B facilitates efficient transcription of protein coding genes. In addition, elongin B interacts with Cullin 2 and von Hippel Lindau tumor suppressor protein to form an E3 ubiquitin ligase complex, which targets hypoxia inducible factor alpha for degradation. By detecting ELOB, researchers can study both transcriptional regulation and proteostasis.\u003cbr\u003e\u003cbr\u003e Western blotting with ELOB antibody detects protein expression in nuclear extracts. Immunohistochemistry maps ELOB distribution in proliferative tissues, while immunofluorescence shows localization in the nucleus and cytoplasm. These assays make ELOB antibody a versatile tool for understanding transcription and protein turnover.\u003cbr\u003e\u003cbr\u003eELOB plays critical roles in hypoxia signaling. The elongin complex with von Hippel Lindau protein mediates degradation of HIF1 alpha under normoxia, maintaining cellular oxygen sensing. Dysregulation of this pathway contributes to tumorigenesis and adaptation to hypoxia. By applying ELOB antibody, scientists can investigate how ubiquitin ligase complexes control oxygen sensing and cancer biology.\u003cbr\u003e\u003cbr\u003eBeyond hypoxia signaling, elongin B supports general protein degradation pathways, acting as a ubiquitin like protein that enhances complex stability. It also contributes to viral replication, as some viruses hijack elongin containing complexes for their own protein turnover. The antibody thus supports studies in virology as well as cell biology.\u003cbr\u003e\u003cbr\u003eMutations or dysregulation of ELOB are linked to cancer progression and altered stress responses. Because of its role in both transcription and protein degradation, elongin B integrates signaling networks that control cell survival and growth. ELOB antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ELOB - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ELOB - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ELOB - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ELOB - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ELOB+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047296393581,"sku":"FY12689","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_783c64c4-a7ee-4d2f-91f0-9bad85303402.jpg?v=1772019327","url":"https:\/\/www.ebiohippo.com\/products\/elob-antibody-elongin-b-bha17135592","provider":"BioHippo","version":"1.0","type":"link"}