{"product_id":"emc1-antibody-er-membrane-protein-complex-subunit-1-bha17135527","title":"EMC1 Antibody \/ ER membrane protein complex subunit 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eEMC1 Antibody \/ ER membrane protein complex subunit 1 is a anti-EMC1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm (ER).\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e EMC1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eEMC1 antibody detects ER membrane protein complex subunit 1, a structural component of the ER membrane protein complex (EMC) responsible for folding and insertion of transmembrane proteins. EMC1 acts as a scaffold stabilizing the entire complex and supports proper biogenesis of multipass membrane proteins. The EMC1 antibody is used in cell biology, proteostasis, and neurodevelopmental research to study membrane protein maturation and ER quality control.\u003cbr\u003e\u003cbr\u003eEMC1 is encoded by the EMC1 gene located on human chromosome 1p36.13. The protein is approximately 993 amino acids long and localizes to the endoplasmic reticulum membrane, where it forms part of a 10-subunit complex including EMC2 through EMC10. EMC1 contains multiple transmembrane segments and luminal domains that interact with nascent polypeptides emerging from the ribosome-translocon system.\u003cbr\u003e\u003cbr\u003eThe EMC1 antibody detects a 115 kilodalton band by western blot and shows reticular ER staining under immunofluorescence microscopy. EMC1 facilitates the co-translational insertion and folding of membrane proteins, including G-protein coupled receptors, ion channels, and transporters. It also interacts with the ER-associated degradation (ERAD) machinery to remove misfolded proteins and prevent aggregation.\u003cbr\u003e\u003cbr\u003eMutations in EMC1 lead to congenital disorders involving neurodevelopmental delay, cerebellar atrophy, and visual impairment, highlighting its importance in neuronal function. In model systems, EMC1 loss disrupts ER morphology and mitochondrial contacts, leading to stress responses and apoptosis. EMC1 also contributes to cholesterol biosynthesis by ensuring proper insertion of enzymes involved in lipid metabolism.\u003cbr\u003e\u003cbr\u003eThrough its essential role in membrane protein biogenesis and ER stability, EMC1 is a cornerstone of cellular proteostasis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=EMC1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=EMC1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=EMC1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/EMC1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=EMC1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047294558573,"sku":"FY12624","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_7d03d08d-7f4f-4a78-b97c-333921c37e53.jpg?v=1782237030","url":"https:\/\/www.ebiohippo.com\/products\/emc1-antibody-er-membrane-protein-complex-subunit-1-bha17135527","provider":"BioHippo","version":"1.0","type":"link"}