{"product_id":"endo-low-plasmid-prepare-kit-bht15200005","title":"Endo-Low Plasmid Prepare Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003ePlasmid Purification Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis kit adopts an improved SDS alkaline lysis method, combined with a high-capacity membrane that selectively adsorbs DNA, for rapid plasmid purification. A dedicated Endo-Remove Buffer step removes endotoxin during purification. It is suitable for extracting up to 60 µg of high-purity plasmid DNA from 5–30 mL of bacterial culture for molecular biology experiments such as sequencing, transfection, in vitro transcription and translation, restriction endonuclease digestion, and bacterial transformation.\u003c\/p\u003e\n\u003cp\u003eBacteria are cultured at 37°C for 12–16 h and harvested by centrifugation, then lysed with Solutions A, B and C2. Endotoxin is removed with Endo-Remove Buffer using a 10 min ice bath followed by 5–10 min at 42°C. After ethanol addition the lysate is bound to the adsorption column, washed and eluted with 100–200 µL of Elution Buffer at 56°C for 5 min. For plasmid DNA obtained with the kit the OD260\/OD280 ratio is about 1.8–2.0.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSpin column (adsorption column P)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eLysis method\u003c\/th\u003e\n\u003ctd\u003eImproved SDS alkaline lysis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e5–30 mL bacterial culture\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eYield\u003c\/th\u003e\n\u003ctd\u003e5–60 µg plasmid DNA per preparation\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEndotoxin removal\u003c\/th\u003e\n\u003ctd\u003eEndo-Remove Buffer treatment step\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eRNA removal\u003c\/th\u003e\n\u003ctd\u003eRNase A supplied\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity (OD260\/OD280)\u003c\/th\u003e\n\u003ctd\u003eAbout 1.8–2.0\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e100–200 µL (at least 100 µL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003eAbout 1 h\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003cth\u003e200 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution A\u003c\/td\u003e\n\u003ctd\u003e25 mL\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution B\u003c\/td\u003e\n\u003ctd\u003e25 mL\u003c\/td\u003e\n\u003ctd\u003e100 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSolution C2\u003c\/td\u003e\n\u003ctd\u003e35 mL\u003c\/td\u003e\n\u003ctd\u003e140 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eWash Buffer\u003c\/td\u003e\n\u003ctd\u003e60 mL\u003c\/td\u003e\n\u003ctd\u003e240 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEndo-Remove Buffer\u003c\/td\u003e\n\u003ctd\u003e9 mL\u003c\/td\u003e\n\u003ctd\u003e36 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eElution Buffer\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003ctd\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase A\u003c\/td\u003e\n\u003ctd\u003e125 µL\u003c\/td\u003e\n\u003ctd\u003e500 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdsorption column (P column)\u003c\/td\u003e\n\u003ctd\u003e50 sets\u003c\/td\u003e\n\u003ctd\u003e200 sets\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePlasmid preparation with endotoxin removal from bacterial culture\u003c\/li\u003e\n\u003cli\u003ePlasmid DNA for sequencing and restriction endonuclease digestion\u003c\/li\u003e\n\u003cli\u003ePlasmid DNA for transfection and cell microinjection\u003c\/li\u003e\n\u003cli\u003eIn vitro transcription and translation\u003c\/li\u003e\n\u003cli\u003eBacterial transformation, ligation and gene silencing experiments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFast and high yield: 5–60 µg plasmids are extracted in about 1 h.\u003c\/li\u003e\n\u003cli\u003eWide range of applications: Suitable for enzyme digestion, PCR, sequencing, ligation, transformation, cell microinjection, gene silencing and transfection.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eSolution A is stored at 4°C for 1 year. Solutions B and C2, Wash Buffer and Elution Buffer are stored at room temperature for 1 year. Endo-Remove Buffer is stored at 2–8°C for 1 year and RNase A at -20°C for 1 year.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMix gently rather than vigorously during lysis and ensure the bacteria are completely lysed before proceeding.\u003c\/li\u003e\n\u003cli\u003eMonitor the pH of Solution C2; it should be no higher than 4.8.\u003c\/li\u003e\n\u003cli\u003eCarry out the centrifugation that follows the Endo-Remove Buffer treatment at 20°C or above.\u003c\/li\u003e\n\u003cli\u003eAdd absolute ethanol to the Wash Buffer before use.\u003c\/li\u003e\n\u003cli\u003eUse at least 100 µL of Elution Buffer and elute at 56°C for 5 min.\u003c\/li\u003e\n\u003cli\u003eThe manufacturer does not publish a residual endotoxin figure for this kit; verify the level in-house if the application requires a defined endotoxin limit.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP015\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200005\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"50 T","offer_id":53965511721325,"sku":"EP015-50T","price":156.0,"currency_code":"USD","in_stock":true},{"title":"200 T","offer_id":53965513884013,"sku":"EP015-200T","price":364.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 5","offer_id":53965513916781,"sku":"EP015-50TX5","price":0.0,"currency_code":"USD","in_stock":true},{"title":"200 T x 5","offer_id":53965513949549,"sku":"EP015-200TX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP015-a-1.jpg?v=1789419189","url":"https:\/\/www.ebiohippo.com\/products\/endo-low-plasmid-prepare-kit-bht15200005","provider":"BioHippo","version":"1.0","type":"link"}