{"product_id":"enhanced-ecl-chemiluminescent-substrate-kit-bht20800098","title":"Enhanced ECL Chemiluminescent Substrate Kit","description":"\u003cdiv class=\"product-block-list__item product-block-list__item--description\"\u003e\n                    \u003cdiv class=\"card\"\u003e\n\u003cdiv class=\"card__header\"\u003e\n                          \u003ch2 class=\"card__title heading h3\"\u003eDescription\u003c\/h2\u003e\n                        \u003c\/div\u003e\n\n                        \u003cdiv class=\"card__section \"\u003e\n                          \u003cdiv class=\"rte text--pull\"\u003e\n                            \u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThe Enhanced ECL Chemiluminescent Substrate Kit is designed for the detection of horseradish peroxidase (HRP)-labeled primary or secondary antibodies and their associated antigens. The principle involves separating proteins or nucleic acids by electrophoresis, transferring them onto a blotting membrane, and then incubating with a primary antibody followed by an HRP-conjugated secondary antibody, or using an HRP-labeled probe to directly or indirectly bind target nucleic acids on the membrane. After washing, the membrane is incubated at room temperature for several minutes with freshly prepared ECL working solution from this kit. The membrane is then wrapped in plastic wrap, placed face-up into an X-ray film cassette, and exposed to X-ray film in a darkroom for seconds to hours. Following development and fixation, protein or nucleic acid bands are clearly visualized on the X-ray film.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\" align=\"justify\"\u003e\u003cspan\u003eThis kit features a unique luminescent substrate system that reduces background signal while incorporating a novel oxidant to significantly enhance stability, allowing the reagent to remain stable for one year at room temperature. In addition to X-ray film detection, the chemiluminescent signal can be directly captured using a fluorescence CCD imager. This product is primarily intended for Western blot (WB) applications and chemiluminescent immunoassay systems.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\"\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eFeatures\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eSuperb Sensitivity\u003c\/span\u003e\u003c\/b\u003e\u003cspan class=\"15\"\u003e: Detects antigens at picogram to low femtogram levels for ultra-sensitive Western blot results.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eHigh Signal-to-Noise Ratio\u003c\/span\u003e\u003c\/b\u003e\u003cspan class=\"15\"\u003e: Advanced luminescent substrates deliver strong signals with minimal background noise.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eAntibody-Efficient\u003c\/span\u003e\u003c\/b\u003e\u003cspan class=\"15\"\u003e: Optimized formulation enhances antibody binding, reducing antibody consumption.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eExcellent Value\u003c\/span\u003e\u003c\/b\u003e\u003cspan class=\"15\"\u003e: Delivers top-tier performance at a competitive price.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cb\u003e\u003cspan class=\"15\"\u003eOutstanding Stability\u003c\/span\u003e\u003c\/b\u003e\u003cspan class=\"15\"\u003e: Improved oxidant system ensures stable storage at 4°C for up to 12 months.\u003c\/span\u003e\u003cspan class=\"15\"\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cstrong\u003eSpecifications\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"1\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eApplicable Samples\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eTarget with high abundance, routine protein samples\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eDetection Sensitivity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eMedium picogram level\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSignal Duration\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e\u0026lt; 2 h\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eApplicable Detection System\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eAll reagents are suitable for X-ray film, CCD imaging devices\u003c\/span\u003e\u003cspan\u003e \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003ePrimary Antibody Dilution Concentration\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1:1000-1:4000\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSecondary Antibody Dilution Concentration\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e1:1000-1:4000\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRecommended Membrane\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eNC membrane\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStorage Stability\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eRoom temperature for 1 year\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eAdvantages\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eStrongest signal among the same level of sensitivity\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"MsoNormal\"\u003e\n\u003cb\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003ctable class=\"MsoTableGrid\" border=\"1\" cellspacing=\"0\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd width=\"176\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eComponents\u003c\/span\u003e\u003cspan\u003e No.\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"427\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eName\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"178\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e36222ES60\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\" valign=\"top\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e36222ES76\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"176\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e36222-A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"427\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSolution A\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"178\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e250 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd width=\"176\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e36222-B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"427\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSolution B\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"178\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e50 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd width=\"179\" valign=\"center\"\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003e250 mL\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3\u003e\n\u003cb\u003e\u003cspan class=\"15\"\u003eStorage\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp\u003eFor frequent use, store at room temperature where it remains stable for up to one year.\u003c\/p\u003e\n\u003cp\u003eFor long-term storage, storing at 4 °C is recommended to extend valid life.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e[Note]:\u003c\/strong\u003e Solution A (36222-A) must be protected from light.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eApplication\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eChemiluminescence ELISA \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eWestern Blot \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eDot Blot-DNA\/RNA \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eSouthern blot-DNA \u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"p\" align=\"justify\"\u003e\u003cspan\u003eNorthern blot-RNA\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 align=\"justify\"\u003e\u003cstrong\u003eFAQ\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003eQ: What is the dilution concentration of the primary antibody used in this product?\u003c\/p\u003e\n\u003cp\u003eA: 1:1000 - 1:4000.\u003c\/p\u003e\n\u003cp\u003eQ: What is the dilution concentration of the secondary antibody used in this product?\u003c\/p\u003e\n\u003cp\u003eA: 1:1000 - 1:4000.\u003c\/p\u003e\n\u003cp\u003eQ: What are the differences between this product and 36208?\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003eA: This product has a high target abundance and a short detection time, making it suitable for routine protein samples. 36208 belongs to a highly sensitive colorimetric kit, with low target abundance, limited samples, and a longer detection time.\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3 class=\"p\"\u003e\n\u003cb\u003e\u003cspan\u003eDocuments\u003c\/span\u003e\u003c\/b\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003cspan\u003eSafety Data Sheet\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003cp\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/36222-MSDS-HB250923.pdf?v=1758679613\"\u003e36222_MSDS_HB250923_EN.PDF\u003c\/a\u003e\u003c\/p\u003e\n\u003cp\u003eManuals\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003ca href=\"https:\/\/cdn.shopify.com\/s\/files\/1\/0803\/9419\/1166\/files\/36222ES-Manual-Ver.EN20250916.pdf?v=1758679611\"\u003e36222_Manual_Ver.EN20250923.pdf\u003c\/a\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch3\u003e\n\u003cstrong\u003eRerference\u003c\/strong\u003e\u003cb\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/b\u003e\n\u003c\/h3\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e\u003cspan\u003e\u003cspan style=\"mso-list: Ignore;\"\u003e1. \u003c\/span\u003e\u003c\/span\u003e\u003c!--[endif]--\u003e\u003cspan\u003eHe X, Li J, Zhou G, et al. Gating of hippocampal rhythms and memory by synaptic plasticity in i\u003c\/span\u003enhibitory interneurons. Neuron. 2021;109(6):1013-1028.e9. doi:10.1016\/j.neuron.2021.01.014. IF = 17.173\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e2. \u003c!--[endif]--\u003eWang X, Zhang S, Jin D, et al. μ-opioid receptor agonist facilitates circulating tumor cell formation in bladder cancer via the MOR\/AKT\/Slug pathway: a comprehensive study including randomized controlled trial. Cancer Commun (Lond). 2023;43(3):365-386. doi:10.1002\/cac2.12408. IF = 16.2\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e3. \u003c!--[endif]--\u003eLi Y, Zhou S, Song H, Yu T, Zheng X, Chu Q. CaCO₃ nanoparticles incorporated with KAE to enable amplified calcium overload cancer therapy. Biomaterials. 2021;277:121080. doi:10.1016\/j.biomaterials.2021.121080. IF = 12.479\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e4. \u003c!--[endif]--\u003eXu Z, Xu J, Sun S, et al. Mecheliolide elicits ROS-mediated ERS-driven immunogenic cell death in hepatocellular carcinoma. Redox Biol. 2022;54:102351. doi:10.1016\/j.redox.2022.102351. IF = 11.799\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e5. \u003c!--[endif]--\u003eCai Z, Zhang Y, Zhang W, et al. Arsenic retention in erythrocytes and excessive erythrophagocytosis is related to low selenium status by impaired redox homeostasis. Redox Biol. 2022;52:102321. doi:10.1016\/j.redox.2022.102321. IF = 11.799\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e6. \u003c!--[endif]--\u003eKaushik AC, Wu Q, Lin L, et al. Exosomal ncRNAs profiling of mycobacterial infection identified miRNA-185-5p as a novel biomarker for tuberculosis. Brief Bioinform. 2021;22(6):bbab210. doi:10.1093\/bib\/bbab210. IF = 11.622\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e7. \u003c!--[endif]--\u003eHu P, Li H, Sun W, et al. Cholesterol-associated lysosomal disorder triggers cell death of hematological malignancy: dynamic analysis on cytotoxic effects of LW-218. Acta Pharm Sin B. 2021;11(10):3178-3192. doi:10.1016\/j.apsb.2021.02.004. IF = 11.614\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e8. \u003c!--[endif]--\u003eWang X, Ni J, You Y, et al. SNX10-mediated LPS sensing causes intestinal barrier dysfunction via a caspase-5-dependent signaling cascade. EMBO J. 2021;40(24):e108080. doi:10.15252\/embj.2021108080. IF = 11.598\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e9. \u003c!--[endif]--\u003eJiang K, Chen H, Fang Y, et al. Exosomal ANGPTL1 attenuates colorectal cancer liver metastasis by regulating Kupffer cell secretion pattern and impeding MMP9-induced vascular leakiness. J Exp Clin Cancer Res. 2021;40(1):21. doi:10.1186\/s13046-020-01816-3. IF = 11.161\u003c\/p\u003e\n\u003cp\u003e\u003c!-- [if !supportLists]--\u003e10. \u003c!--[endif]--\u003eZhang S, Yang Z, Bao W, et al. SNX10 (sorting nexin 10) inhibits colorectal cancer initiation and progression by controlling autophagic degradation of SRC. Autophagy. 2020;16(4):735-749. doi:10.1080\/15548627.2019.1632122. IF = 11.059\u003c\/p\u003e\n\u003cp class=\"MsoNormal\"\u003e\u003c!-- [if !supportLists]--\u003e11. \u003c!--[endif]--\u003eLiu L, Liang L, Yang C, Zhou Y, Chen Y. Extracellular vesicles of Fusobacterium nucleatum compromise intestinal barrier through targeting RIPK1-mediated cell death pathway. Gut Microbes. 2021;13(1):1-20. doi:10.1080\/19490976.2021.1902718. 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