{"product_id":"eno1-antibody-enolase-1-alpha-enolase-bha17136282","title":"ENO1 Antibody \/ Enolase 1 \/ Alpha Enolase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eENO1 Antibody \/ Enolase 1 \/ Alpha Enolase is a anti-ENO1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ENO1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eENO1 antibody detects Enolase 1, also known as alpha-enolase, a glycolytic enzyme encoded by the ENO1 gene on chromosome 1p36.23. ENO1 catalyzes the reversible dehydration of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway and serves multiple non-glycolytic roles, including acting as a plasminogen-binding receptor on the cell surface. ENO1 is ubiquitously expressed in most tissues, with high levels in brain, muscle, and epithelial cells, where it contributes to energy metabolism and cellular adaptation under hypoxic conditions.\u003cbr\u003e\u003cbr\u003eStructurally, ENO1 is a 48 kDa homodimeric enzyme that belongs to the enolase family of metalloenzymes. It requires magnesium ions for catalytic activity and forms part of the glycolytic enzyme network in the cytosol. ENO1 also functions as a moonlighting protein, existing in different subcellular locations and performing distinct functions. In addition to its cytoplasmic role, a nuclear isoform, MBP-1 (Myc-binding protein 1), acts as a transcriptional repressor of the oncogene MYC. Surface-localized ENO1 binds plasminogen, promoting extracellular matrix degradation and cell migration.\u003cbr\u003e\u003cbr\u003eFunctionally, ENO1 plays a dual role in metabolism and cellular regulation. In glycolysis, it facilitates ATP generation by catalyzing one of the final steps of the pathway. On the plasma membrane, it promotes cell migration and invasion by enhancing plasmin-mediated proteolysis. The MBP-1 isoform contributes to tumor suppression by downregulating MYC expression. ENO1 also acts as a stress response protein, supporting cell survival under hypoxic and oxidative stress conditions. Known ligands include plasminogen and structural components of the cytoskeleton, such as actin and tubulin.\u003cbr\u003e\u003cbr\u003eAberrant expression of ENO1 has been linked to cancer, autoimmune disease, and neurodegenerative disorders. Overexpression supports the Warburg effect in tumor metabolism, whereas autoantibodies against ENO1 have been detected in Hashimoto�s encephalopathy and systemic autoimmune diseases. Pathway involvement includes glycolysis, gluconeogenesis, and plasminogen activation. During embryonic development, ENO1 expression supports rapid cell proliferation and differentiation.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using ENO1 antibody reveals cytoplasmic and membranous localization in epithelial cells and neurons. The ENO1 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ENO1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ENO1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ENO1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ENO1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ENO1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047324442989,"sku":"FY13380","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_5e17fe75-dbba-482a-81e7-6421039aa88b.jpg?v=1782237116","url":"https:\/\/www.ebiohippo.com\/products\/eno1-antibody-enolase-1-alpha-enolase-bha17136282","provider":"BioHippo","version":"1.0","type":"link"}