{"product_id":"enterokinase-cleavage-enzyme-mammalian-produced-bhz10900163","title":"Enterokinase Cleavage Enzyme (Mammalian Produced)","description":"\u003cdiv id=\"answer\"\u003e\n\u003cstrong\u003eEnterokinase Cleavage Enzyme\u003c\/strong\u003e (EK1) is a highly pure and active endotoxin-free enterokinase that is produced using human cell line in serum free media. It is highly specific for cleaving fusion proteins with the recognition sequence, Asp-Asp-Asp-Asp-Lys (DDDDK). Because it is produced using human cell line, it has the appropriate glycosylation to catalyze highly accurate cleavage reaction with minimum non-specific activity. Its activity is stable over a broad temperature range, and it completes the reaction from 4°C to 60°C.\u003c\/div\u003e\n\u003cdiv\u003e \u003c\/div\u003e\n\u003cdiv\u003eUnlike other site-specific proteases that cut within the recognition sequences leaving extra amino acids in the cleaved peptide products, the EK1 cleaves after the C-terminal end of the lysine residue, and the fragment produced from the cleavage reaction does not inherit any residues from the DDDDK recognition sequence. Therefore, the application can be extremely advantageous for producing a 100% native protein sequence and structure from recombinant fusion protein, which has the desired product immediately after the enterokinase recognition sequence, DDDDK.\u003c\/div\u003e\n\u003cdiv\u003e \u003c\/div\u003e\n\u003cdiv\u003eOwing to the presence of a His tag , EK1 can be easily removed after the cleavage reaction by affinity chromatography with Ni-IDA Agarose Beads. In addition, DDDDK is a part of the octapeptide FLAG tag (DYKDDDDK), which can be utilized as a fusion tag for recognition by antibody, and for detection of fusion protein expression with Western blot analysis, as well as for purification of the fusion protein by Anti-FLAG affinity chromatography. This array of applications makes EK1 an ideal tool in the research involving the study of protein structure and function, and protein production where native protein structures and sequences are desired.\u003c\/div\u003e\n\u003cdiv\u003e \u003c\/div\u003e\n\u003cdiv id=\"question\"\u003e\u003cstrong\u003eSpecial Features\u003c\/strong\u003e\u003c\/div\u003e\n\u003cdiv id=\"answer\"\u003e\n\u003cul\u003e\n\u003cli\u003eEndotoxin free (i.e., produced in mammalian cells and purified from serum free media)\u003c\/li\u003e\n\u003cli\u003elycosylated for improved efficiency and stability\u003c\/li\u003e\n\u003cli\u003eRapid cleavage (95% cleavage in 1hr)\u003c\/li\u003e\n\u003cli\u003eHighly specific with minimum non-specific cleavage (even at 20x concentration)\u003c\/li\u003e\n\u003cli\u003eStable over broad temperature range\u003c\/li\u003e\n\u003cli\u003eHis-tagged for convenient removal post reaction\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c\/div\u003e\u003ctable class=\"bhc-specs-table\" style=\"width:100%;border-collapse:collapse\"\u003e\n\u003cthead\u003e\u003ctr style=\"background:#1a5c58;color:#fff\"\u003e\n\u003cth style=\"padding:8px 12px;text-align:left\"\u003eSpecification\u003c\/th\u003e\n\u003cth style=\"padding:8px 12px;text-align:left\"\u003eValue\u003c\/th\u003e\n\u003c\/tr\u003e\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr style=\"border-bottom:1px solid #d9d9d9\"\u003e\n\u003ctd style=\"padding:8px 12px;font-weight:600;color:#1b2f42;width:40%\"\u003eEnzyme Type\u003c\/td\u003e\n\u003ctd style=\"padding:8px 12px\"\u003eProtease\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #d9d9d9\"\u003e\n\u003ctd style=\"padding:8px 12px;font-weight:600;color:#1b2f42;width:40%\"\u003eFormat\u003c\/td\u003e\n\u003ctd style=\"padding:8px 12px\"\u003eEnzyme Only\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr style=\"border-bottom:1px solid #d9d9d9\"\u003e\n\u003ctd style=\"padding:8px 12px;font-weight:600;color:#1b2f42;width:40%\"\u003eStorage Conditions\u003c\/td\u003e\n\u003ctd style=\"padding:8px 12px\"\u003eWe recommend that the vial be briefly centrifuged prior to opening to bring the contents to the bottom. The stock solutions should be distributed into working aliquots and store at -20°C. Avoid multiple freeze\/thaw cycles. Stable for 3 years from the date of shipping when stored and handled as per instructions.\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"100 Units","offer_id":53294164279661,"sku":"G699","price":118.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/GQuS5IENNjS8FKhZVaZ9RDEJdJjjhosxyNAIrYo6.png?v=1778484903","url":"https:\/\/www.ebiohippo.com\/products\/enterokinase-cleavage-enzyme-mammalian-produced-bhz10900163","provider":"BioHippo","version":"1.0","type":"link"}