{"product_id":"entilink-1st-strand-cdna-synthesis-kit-bht15200031","title":"EntiLink™ 1st Strand cDNA Synthesis Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003ecDNA Synthesis Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThe kit contains all the components needed to synthesize high-quality first-strand cDNA from total RNA or mRNA, and provides two primers for cDNA synthesis: Random Primers N6 and oligo (dT)18. The synthesized single-stranded cDNA product can be used directly in subsequent PCR or qPCR reactions.\u003c\/p\u003e\n\u003cp\u003eFirst-strand cDNA is generated from 1 ng–5 µg of total RNA or 1 ng–500 ng of mRNA, and the cDNA produced can be amplified up to 10 kb. For qPCR the manual recommends no more than 1 µg of total RNA or 100 ng of mRNA per reaction. Reverse transcription is carried out in a 20 µL system at 25°C for 5 min, 42°C for 30 min and 85°C for 5 min, and the enzyme mix contains an RNase inhibitor.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eReagent kit for first-strand cDNA synthesis\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStarting material\u003c\/th\u003e\n\u003ctd\u003eTotal RNA or mRNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input, total RNA\u003c\/th\u003e\n\u003ctd\u003e1 ng–5 µg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input, mRNA\u003c\/th\u003e\n\u003ctd\u003e1 ng–500 ng\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProduct use\u003c\/th\u003e\n\u003ctd\u003eFull-length cDNA amplifiable up to 10 kb\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReaction volume\u003c\/th\u003e\n\u003ctd\u003e20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReactions per kit\u003c\/th\u003e\n\u003ctd\u003e100 rxn\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e100 rxn\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-Free ddH2O\u003c\/td\u003e\n\u003ctd\u003e2 × 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e5× Buffer\u003c\/td\u003e\n\u003ctd\u003e400 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEntiLink™ Enzyme Mix\u003c\/td\u003e\n\u003ctd\u003e200 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eOligo (dT)18 (50 µM)\u003c\/td\u003e\n\u003ctd\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRandom Primers N6 (50 µM)\u003c\/td\u003e\n\u003ctd\u003e100 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eFirst-strand cDNA synthesis from total RNA or mRNA\u003c\/li\u003e\n\u003cli\u003eTemplate preparation for PCR\u003c\/li\u003e\n\u003cli\u003eTemplate preparation for qPCR\u003c\/li\u003e\n\u003cli\u003eReverse transcription of RNA templates with complex secondary structures\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eThe EntiLink™ Reverse Transcriptase has dramatically improved thermal stability and can withstand reaction temperatures up to 50°C, making it suitable for reverse transcription of RNA templates with complex secondary structures.\u003c\/li\u003e\n\u003cli\u003eThe EntiLink™ Reverse Transcriptase also has an increased affinity for templates and is suitable for reverse transcription of small amounts of templates and low-copy genes.\u003c\/li\u003e\n\u003cli\u003eThe EntiLink™ Reverse Transcriptase also has an improved ability to synthesize full-length cDNAs, which can be amplified up to 10 kb in length.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eAll components are stored at -20°C. Synthesized cDNA is stored at -20°C for short-term storage and at -80°C for long-term storage.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ePerform operations on ice.\u003c\/li\u003e\n\u003cli\u003eAvoid RNase contamination and use RNase-free tubes, filter-tip pipettes, gloves and masks.\u003c\/li\u003e\n\u003cli\u003eStore cDNA products at -20°C for short-term storage and at -80°C for long-term storage.\u003c\/li\u003e\n\u003cli\u003eFor qPCR, use no more than 1 µg of total RNA or 100 ng of mRNA per reaction.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEQ003\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200031\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"100 rxn","offer_id":53965513261421,"sku":"EQ003-100RXN","price":364.0,"currency_code":"USD","in_stock":true},{"title":"100 rxn x 5","offer_id":53965514309997,"sku":"EQ003-100RXNX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EQ003-a-1.jpg?v=1789419129","url":"https:\/\/www.ebiohippo.com\/products\/entilink-1st-strand-cdna-synthesis-kit-bht15200031","provider":"BioHippo","version":"1.0","type":"link"}