{"product_id":"entpd5-antibody-ectonucleoside-triphosphate-diphosphohydrolase-5-bha17136161","title":"ENTPD5 Antibody \/ Ectonucleoside triphosphate diphosphohydrolase 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eENTPD5 Antibody \/ Ectonucleoside triphosphate diphosphohydrolase 5 is a anti-ENTPD5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: ER.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ENTPD5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eENTPD5 antibody detects Ectonucleoside triphosphate diphosphohydrolase 5, an endoplasmic reticulum (ER)-localized enzyme that regulates protein N-glycosylation and energy metabolism. The UniProt recommended name is Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5). Unlike other ENTPD family members that function extracellularly, ENTPD5 acts within the ER lumen, promoting protein folding and glycoprotein maturation through its unique enzymatic cycle.\u003cbr\u003e\u003cbr\u003eFunctionally, ENTPD5 antibody identifies a 428-amino-acid enzyme that hydrolyzes UDP and GDP to UMP and GMP, coupling its activity to the UDP-glucose pyrophosphorylase reaction that regenerates UDP-glucose. This cycle supports proper glycoprotein processing by maintaining a continuous supply of glycosylation precursors within the ER. ENTPD5 activity enhances protein folding capacity and assists in the maturation of membrane receptors and secretory proteins.\u003cbr\u003e\u003cbr\u003eThe ENTPD5 gene is located on chromosome 14q24.1 and is expressed in metabolically active tissues such as liver, pancreas, and muscle. Its expression is transcriptionally upregulated by AKT signaling and MYC oncogene activation, linking metabolic reprogramming to cancer cell growth. ENTPD5 contributes to the Warburg effect by coupling glycosylation demand to glycolytic flux in proliferating cells.\u003cbr\u003e\u003cbr\u003ePathologically, overexpression of ENTPD5 promotes tumor progression by increasing glycoprotein folding and surface receptor stability, enhancing proliferative signaling. Conversely, loss of ENTPD5 impairs growth factor receptor maturation and induces ER stress. Elevated ENTPD5 expression has been observed in prostate, colon, and liver cancers. Research using ENTPD5 antibody supports studies in cancer metabolism, glycoprotein folding, and ER homeostasis.\u003cbr\u003e\u003cbr\u003eENTPD5 antibody is validated for western blotting, immunohistochemistry, and ELISA to detect ER-localized glycoprotein processing enzymes. \u003cbr\u003e\u003cbr\u003eStructurally, Ectonucleoside triphosphate diphosphohydrolase 5 contains an apyrase conserved region (ACR) and calcium-binding motifs essential for nucleotide hydrolysis. It forms dimers in the ER lumen and associates with chaperones such as calnexin and calreticulin. This antibody enables exploration of ENTPD5's role in glycoprotein biosynthesis, ER stress adaptation, and cancer metabolism.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ENTPD5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ENTPD5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ENTPD5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ENTPD5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ENTPD5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047319724397,"sku":"FY13259","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_2c8d60b2-0dce-4293-8afd-9b0771474bb7.jpg?v=1782237089","url":"https:\/\/www.ebiohippo.com\/products\/entpd5-antibody-ectonucleoside-triphosphate-diphosphohydrolase-5-bha17136161","provider":"BioHippo","version":"1.0","type":"link"}