{"product_id":"enturbo-sybr-color-qpcr-supermix-bht15200038","title":"EnTurbo™ SYBR Color qPCR SuperMix","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eqPCR Master Mixes\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eEnTurbo™ SYBR Color qPCR SuperMix is an optimized 2× Real-time PCR premix containing HotStarTaq DNA Polymerase, SYBR Green I fluorochrome dye, dNTP and Mg2+. In addition, the balanced K+ and NH4+ ion ratios in the buffer promote specific primer annealing. To ensure a highly sensitive and specific PCR reaction, the reaction can be initiated by simply adding the primer and cDNA template to the ready-to-use PCR master premix. The unique PCR buffer ensures sensitive qPCR on all real-time PCR instruments without optimization.\u003c\/p\u003e\n\u003cp\u003eIn addition, the product visualizes the pipetting process through the colour change reaction after the template is added, which greatly improves pipetting efficiency and avoids pipetting errors. A stand-alone 50× ROX Dye is supplied and is added at 0.4–2 µL per 20 µL system depending on the instrument. The blue dye in the mix and the yellow dye in the 10 × Dilution Buffer generate a blue-to-green colour change, and the HotStarTaq DNA Polymerase is activated at 95°C for 30 sec. A 20 µL reaction contains 10 µL of 2× mix, 0.4 µL of each 10 µM primer, 0.4–2 µL of 50× ROX Dye, template and ddH2O, and is run at 95°C for 30 sec, then 35–40 cycles of 95°C for 5 sec and 60°C for 30 sec (two-step; the three-step program uses separate 50–60°C annealing and 72°C extension steps), followed by melt curve analysis.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e2× real-time PCR premix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePolymerase\u003c\/th\u003e\n\u003ctd\u003eHotStarTaq DNA Polymerase (hot start, activated at 95°C for 30 sec)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection chemistry\u003c\/th\u003e\n\u003ctd\u003eSYBR Green I fluorochrome dye\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eROX\u003c\/th\u003e\n\u003ctd\u003eStand-alone 50× ROX Dye, 1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eColour tracking\u003c\/th\u003e\n\u003ctd\u003eBlue-to-green colour change (blue dye in mix, yellow dye in buffer)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate input, cDNA\u003c\/th\u003e\n\u003ctd\u003e10 pg to 1 ng total RNA per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate input, genomic DNA\u003c\/th\u003e\n\u003ctd\u003e100 pg to 1 ng per 20 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate input, plasmid DNA\u003c\/th\u003e\n\u003ctd\u003e10¹–10⁷ copies per 20 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReaction volume\u003c\/th\u003e\n\u003ctd\u003e20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e20 µL × 500 rxn\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2 × SYBR Color qPCR Mix\u003c\/td\u003e\n\u003ctd\u003e4 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 × Dilution Buffer\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e50 × ROX Dye\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eddH2O\u003c\/td\u003e\n\u003ctd\u003e4 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGene expression analysis of cDNA\u003c\/li\u003e\n\u003cli\u003eAbsolute quantification of plasmids\u003c\/li\u003e\n\u003cli\u003eAbsolute quantification of genomic DNA\u003c\/li\u003e\n\u003cli\u003eQuantification of sequencing libraries\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eQuickly get results, saving up to 50% of the time\u003c\/li\u003e\n\u003cli\u003eOptimized ready-to-use master premix for rapid PCR reactions\u003c\/li\u003e\n\u003cli\u003eAccurate detection of various starting amounts of template, stable amplification quantitative results with high repeatability\u003c\/li\u003e\n\u003cli\u003eBalanced K+ and NH4+ ion ratios ensure high sensitivity and specificity\u003c\/li\u003e\n\u003cli\u003eStand-alone ROX Reference Dye package for all Real-time PCR instruments\u003c\/li\u003e\n\u003cli\u003eReduces pipetting errors by tracking the pipetting process through the color change reaction between dyes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStored at -20°C in the dark with a shelf life of one year. Avoid strong light during storage. Shipping: Transport on ice or dry ice.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eWear a lab coat and disposable gloves when performing the experiment.\u003c\/li\u003e\n\u003cli\u003eAvoid strong light during storage.\u003c\/li\u003e\n\u003cli\u003eMix by inversion before use.\u003c\/li\u003e\n\u003cli\u003eUse gradient dilution for high-abundance genes to improve accuracy.\u003c\/li\u003e\n\u003cli\u003eUse at least three biological replicates.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEQ036\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200038\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"5 mL","offer_id":53965512343917,"sku":"EQ036-5ML","price":182.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":53965517422957,"sku":"EQ036-25ML","price":728.0,"currency_code":"USD","in_stock":true},{"title":"5 mL x 10","offer_id":53965517455725,"sku":"EQ036-5MLX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"25 mL x 4","offer_id":53965517488493,"sku":"EQ036-25MLX4","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EQ036-a-1.jpg?v=1789419182","url":"https:\/\/www.ebiohippo.com\/products\/enturbo-sybr-color-qpcr-supermix-bht15200038","provider":"BioHippo","version":"1.0","type":"link"}