{"product_id":"enturbo-sybr-color-qpcr-supermix-low-rox-premixed-bht15200043","title":"EnTurbo™ SYBR Color qPCR SuperMix (Low ROX Premixed)","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eqPCR Master Mixes\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eEnTurbo™ SYBR Color qPCR SuperMix is an optimized 2× real-time PCR master mix containing HotStarTaq DNA Polymerase, SYBR Green® fluorescent dye, dNTP and Mg2+, Low ROX. In addition, the balanced K+ and NH4+ ion ratios in the buffer promote specific primer annealing. To ensure a highly sensitive and specific PCR reaction, the reaction can be initiated by simply adding the primer and cDNA template to the ready-to-use PCR master mix. This simplifies the workflow and reduces the risk of contamination. The unique PCR buffer ensures sensitive qPCR.\u003c\/p\u003e\n\u003cp\u003eIn addition, the product realizes the visualization of the sample addition process through the discoloration reaction after the template is added, which greatly improves the sample addition efficiency and avoids sample addition errors. A blue-to-green colour change indicates that template has been added. Per 20 µL reaction the manual specifies 10 pg–1 ng of cDNA, 10¹–10⁷ copies of plasmid DNA or 100 pg–1 ng of genomic DNA. A 20 µL reaction contains 10 µL of master mix, 0.4 µL of each primer, template and ddH2O, and is run at 95°C for 30 sec, then 35–40 cycles of 95°C for 5 sec and 60°C for 30 sec, followed by melting curve analysis.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e2× real-time PCR master mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePolymerase\u003c\/th\u003e\n\u003ctd\u003eHotStarTaq DNA Polymerase (hot start)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection chemistry\u003c\/th\u003e\n\u003ctd\u003eSYBR Green dye\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eROX\u003c\/th\u003e\n\u003ctd\u003eLow ROX premixed\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eColour tracking\u003c\/th\u003e\n\u003ctd\u003eBlue-to-green colour change indicates template addition\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate input, cDNA\u003c\/th\u003e\n\u003ctd\u003e10 pg–1 ng per 20 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate input, plasmid DNA\u003c\/th\u003e\n\u003ctd\u003e10¹–10⁷ copies per 20 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate input, genomic DNA\u003c\/th\u003e\n\u003ctd\u003e100 pg–1 ng per 20 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReaction volume\u003c\/th\u003e\n\u003ctd\u003e20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e20 µL × 500 rxn\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e2 × SYBR Color qPCR Mix (Low ROX)\u003c\/td\u003e\n\u003ctd\u003e4 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10 × Dilution Buffer\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eddH2O\u003c\/td\u003e\n\u003ctd\u003e4 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003ecDNA gene expression analysis\u003c\/li\u003e\n\u003cli\u003ePlasmid quantification\u003c\/li\u003e\n\u003cli\u003eQuantification of genomic DNA\u003c\/li\u003e\n\u003cli\u003eQuantification of sequencing libraries\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eQuickly get results, saving up to 50% of the time\u003c\/li\u003e\n\u003cli\u003eOptimized ready-to-use premix for rapid PCR reactions\u003c\/li\u003e\n\u003cli\u003eAccurate detection of various starting amounts of templates, stable amplification, quantitative results with high repeatability\u003c\/li\u003e\n\u003cli\u003eBalanced K+ and NH4+ ion ratios, ensure high sensitivity and high specificity\u003c\/li\u003e\n\u003cli\u003eTrack the pipetting process and reduce pipetting errors through the color change reaction between dyes\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStored at -20°C with a shelf life of one year. Avoid strong light during storage and reaction setup.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAvoid strong light during storage and reaction setup.\u003c\/li\u003e\n\u003cli\u003eWear a lab coat and gloves when performing the experiment.\u003c\/li\u003e\n\u003cli\u003eUse at least three biological replicates.\u003c\/li\u003e\n\u003cli\u003eIncrease the pre-denaturation step to 2 min for high-GC or complex templates.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEQ033\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200043\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"5 mL","offer_id":53965512966509,"sku":"EQ033-5ML","price":182.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":53965516145005,"sku":"EQ033-25ML","price":728.0,"currency_code":"USD","in_stock":true},{"title":"5 mL x 10","offer_id":53965516177773,"sku":"EQ033-5MLX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"25 mL x 4","offer_id":53965516210541,"sku":"EQ033-25MLX4","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EQ033-a-1.jpg?v=1789419310","url":"https:\/\/www.ebiohippo.com\/products\/enturbo-sybr-color-qpcr-supermix-low-rox-premixed-bht15200043","provider":"BioHippo","version":"1.0","type":"link"}