{"product_id":"enturbo-sybr-green-pcr-supermix-bht15200036","title":"EnTurbo™ SYBR Green PCR SuperMix","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eqPCR Master Mixes\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eEnTurbo™ SYBR Green PCR SuperMix is an optimized 2× real-time PCR master mix containing HotStarTaq DNA Polymerase, SYBR Green® fluorescent dye, dNTP and Mg2+. In addition, the balanced K+ and NH4+ ion ratios in the buffer promote specific primer annealing. To ensure a highly sensitive and specific PCR reaction, the reaction can be initiated by simply adding the primer and cDNA template to the ready-to-use PCR master mix. The unique PCR buffer ensures sensitive qPCR on all real-time PCR instruments without optimization.\u003c\/p\u003e\n\u003cp\u003eThe documented pack is 20 µL × 500 rxn, which corresponds to the 5 mL option and contains 4 × 1.25 mL of SuperMix with a stand-alone 50× ROX Dye and RNase-Free ddH2O; it is used for gene expression analysis and absolute quantitative analysis. Input per 20 µL reaction is 10 pg–1 ng of cDNA; for plasmid or genomic DNA the input is 100 pg–1 ng, or 10–10⁷ copies. The manual reports a Ct value range of 5–35, single-digit copy detection and run time reduced by 50%.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eConcentration\u003c\/th\u003e\n\u003ctd\u003e2× real-time PCR master mix\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePolymerase\u003c\/th\u003e\n\u003ctd\u003eHotStarTaq DNA Polymerase (hot start)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection chemistry\u003c\/th\u003e\n\u003ctd\u003eSYBR Green® fluorescent dye\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eROX\u003c\/th\u003e\n\u003ctd\u003eStand-alone 50× ROX Dye supplied separately\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate input, cDNA\u003c\/th\u003e\n\u003ctd\u003e10 pg–1 ng per reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eTemplate input, plasmid or genomic DNA\u003c\/th\u003e\n\u003ctd\u003e100 pg–1 ng, or 10–10⁷ copies per 20 µL reaction\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eReaction volume\u003c\/th\u003e\n\u003ctd\u003e20 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCt value range\u003c\/th\u003e\n\u003ctd\u003e5–35\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSensitivity\u003c\/th\u003e\n\u003ctd\u003eSingle-digit copy detection\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e20 µL × 500 rxn\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eEnTurbo™ SYBR Green PCR SuperMix\u003c\/td\u003e\n\u003ctd\u003e4 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e50× ROX Dye\u003c\/td\u003e\n\u003ctd\u003e1 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-Free ddH2O\u003c\/td\u003e\n\u003ctd\u003e4 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eGene expression analysis\u003c\/li\u003e\n\u003cli\u003eAbsolute quantitative analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eQuickly get results, saving up to 50% of the time\u003c\/li\u003e\n\u003cli\u003eOptimized ready-to-use master mix for rapid PCR reactions\u003c\/li\u003e\n\u003cli\u003eAccurate detection of various starting amounts of templates, stable amplification, quantitative results with high repeatability\u003c\/li\u003e\n\u003cli\u003eBalanced K+ and NH4+ ion ratios, as well as stand-alone ROX Reference Dye packaging for all real-time PCR instruments\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eStored at -20°C with a shelf life of one year. Avoid strong light during storage.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eAvoid strong light during storage.\u003c\/li\u003e\n\u003cli\u003eWear a lab coat and disposable gloves when performing the experiment.\u003c\/li\u003e\n\u003cli\u003eMix by inversion before use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEQ001\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200036\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"5 mL","offer_id":53965512671597,"sku":"EQ001-5ML","price":182.0,"currency_code":"USD","in_stock":true},{"title":"25 mL","offer_id":53965514932589,"sku":"EQ001-25ML","price":728.0,"currency_code":"USD","in_stock":true},{"title":"5 mL x 10","offer_id":53965514965357,"sku":"EQ001-5MLX10","price":0.0,"currency_code":"USD","in_stock":true},{"title":"25 mL x 4","offer_id":53965514998125,"sku":"EQ001-25MLX4","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EQ001-a-1.jpg?v=1789419186","url":"https:\/\/www.ebiohippo.com\/products\/enturbo-sybr-green-pcr-supermix-bht15200036","provider":"BioHippo","version":"1.0","type":"link"}