{"product_id":"enzychrom-monoamine-oxidase-inhibitor-screening-kit-bht15600176","title":"EnzyChrom™ Monoamine Oxidase Inhibitor Screening Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor evaluation and high-throughput screen (HTS) of monoamine oxidase modulators. The assay uses FL530\/585nm for signal readout. Compatible sample input includes Compounds that affect monoamine oxidase (MAO) activity. Typical stated assay timing is 35 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e FL530\/585nm supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Compounds that affect monoamine oxidase (MAO) activity, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eWorkflow timing:\u003c\/strong\u003e The listed assay time of 35 min helps frame batch planning, replicate handling, and plate throughput.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Homogeneous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests (Custom bulk sizes available upon request).\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of monoamine oxidase inhibitor screening within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003e\u003cem\u003eMONOAMINE OXIDASES\u003c\/em\u003e(MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. Two isoforms of MAO exist, MAO-A and MAO-B, with different inhibitor selectivity and tissue distribution. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAOs in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drugs prescribed for the treatment of depression, Parkinson’s, and Alzheimer’s diseases. BioAssay Systems MAO Inhibitor Screening Assay Kit provides a convenient fluorimetric means to screen for MAO enzyme inhibitors. In the assay, MAO reacts with\u003cem\u003ep\u003c\/em\u003e-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003e, which is determined by a fluorimetric method (λ\u003csub\u003eem\/ex\u003c\/sub\u003e= 585\/530 nm). The assay is simple, sensitive, stable, and high-throughput adaptable.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eFluorescent (FL 530\/585 nm).\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 35 min.\u003c\/p\u003e\n\n\u003ch2\u003eScreening services\u003c\/h2\u003e\n\u003cp\u003eBioAssay Systems offers comprehensive Monoamine Oxidase lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service .\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eThe product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.\u003c\/li\u003e\n  \u003cli\u003eThe description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify monoamine oxidase inhibitor screening in compounds that affect monoamine oxidase (MAO) by FL530\/585 nm readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched compounds that affect monoamine oxidase (MAO) handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in compounds that affect monoamine oxidase (MAO) across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests (Custom bulk sizes available upon request)","offer_id":53238318629229,"sku":"EIMO-100","price":459.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EIMOfig.jpg?v=1776668360","url":"https:\/\/www.ebiohippo.com\/products\/enzychrom-monoamine-oxidase-inhibitor-screening-kit-bht15600176","provider":"BioHippo","version":"1.0","type":"link"}