{"product_id":"enzychrom-uric-acid-assay-kit-ii-bht15600214","title":"EnzyChrom™ Uric Acid Assay Kit II","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eUric acid determination in biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts. Sensitive. Use 10 µL samples. Linear detection range: Colorimetric assay 15 - 1000 µM Uric Acid. Fluorimetric. The assay uses OD570 or FL530\/585nm for signal readout. Compatible sample input includes Biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts.. Typical stated assay timing is 30 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e OD570 or FL530\/585nm supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts., which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAnalytical range context:\u003c\/strong\u003e The supplied specifications include a stated detection limit of 4 μM for interpreting low-signal samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Sensitive. Use 10 μL samples. Linear detection range: Colorimetric assay 15 – 1000 μM Uric Acid. Fluorimetric assay 4 – 300 μM Uric Acid.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Fast. Run time is 30 minutes for Optical Density method and 10 minutes for Fluorimetric method for rapid results; Convenient. Room temperature “mix-and-read” procedure can be readily automated for high-throughput assay of thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of uric acid within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003eURIC ACID\u003c\/i\u003eis the breakdown product of purines such as adenosine and inosine. Humans and other primates are incapable of further metabolizing uric acid. In humans, uric acid is excreted via the kidneys, but a failure to remove excess uric acid can lead to conditions such as gout or uric acid kidney stones.BioAssay Systems’ method provides a simple and high-throughput assay for measuring uric acid levels. In this assay, uric acid is enzymatically converted to allantoin, releasing H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003e. The resulting H\u003csub\u003e2\u003c\/sub\u003eO\u003csub\u003e2\u003c\/sub\u003ereacts with a specific dye to form a pink-colored product. The change in OD570nm or fluorescence intensity at λex\/em = 530\/585nm is directly proportional to the uric acid present in the sample.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eColorimetric (OD 570 nm) or Fluorescent (FL 530\/585 nm).\u003c\/p\u003e\n\n\u003ch2\u003eDetection limit and analytical sensitivity\u003c\/h2\u003e\n\u003cp\u003eReported detection limit: 4 μM.\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 30 min.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eThe product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.\u003c\/li\u003e\n  \u003cli\u003eShort assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify uric acid in serum, urine by OD570 or FL530\/585 nm readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched serum, urine handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in serum, urine across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests in 96-well plate","offer_id":53238320791917,"sku":"EUAC-100","price":469.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EUACfig.jpg?v=1776668364","url":"https:\/\/www.ebiohippo.com\/products\/enzychrom-uric-acid-assay-kit-ii-bht15600214","provider":"BioHippo","version":"1.0","type":"link"}