{"product_id":"enzyfluo-erk-phosphorylation-assay-kit-bht15600144","title":"EnzyFluo™ ERK Phosphorylation Assay Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor quantitative fluorescent immunoenzymatic assay of ERK1\/2 phosphorylation status in cultured cells. The assay uses Cell-Based ELISA (FL360\/450nm, 530\/585nm) for signal readout. Compatible sample input includes Cells. Typical stated assay timing is Assay takes 6.5 hrs, hands-on time 2.5 hrs.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e Cell-Based ELISA (FL360\/450nm, 530\/585nm) supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Cells, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eWorkflow timing:\u003c\/strong\u003e The listed assay time of Assay takes 6.5 hrs, hands-on time 2.5 hrs helps frame batch planning, replicate handling, and plate throughput.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis is necessary; Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of enzyfluo erk phosphorylation within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003eThe\u003ci\u003emitogen-activated protein kinase \u003c\/i\u003e(MAPK\/ERK) pathway plays a key role in cell proliferation, differentiation, and migration. Stimulation by mitogens eventually leads to phosphorylation of ERK1 (T202\/Y204) and ERK2 (T185\/Y187). The MAPK\/ERK cascade presents many interesting drug targets for the development of cancer therapies. BioAssay Systems cell-based ELISA measures dually phosphorylated ERK1\/2 in whole cells and normalizes the signal to the total protein content. This simple and efficient assay eliminates the need for cell lysate preparation and can be used to study kinase signaling and the effects of kinase inhibitors on cells. In this assay, cells are grown in 96-well plates and treated with ligands or drugs. Cells are then fixed and permeabilized in the wells. ERK1\/2 phosphorylation (pERK) using a fluorescent ELISA followed by total protein measurement in each well.\u003cbr class=\"\"\u003e\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eCell-Based ELISA (FL360\/450nm, 530\/585nm).\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: Assay takes 6.5 hrs, hands-on time 2.5 hrs.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eThe product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.\u003c\/li\u003e\n  \u003cli\u003eShort assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify enzyfluo erk phosphorylation in cells by Cell-Based ELISA (FL360\/450 nm, 530\/585 nm) readout.\u003c\/li\u003e\n  \u003cli\u003eCompare treatment or phenotype groups using matched cells handling.\u003c\/li\u003e\n  \u003cli\u003eMonitor time-course or pre\/post changes in cells across study conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests","offer_id":53238316728685,"sku":"EERK-100","price":599.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EERKfig.jpg?v=1776668354","url":"https:\/\/www.ebiohippo.com\/products\/enzyfluo-erk-phosphorylation-assay-kit-bht15600144","provider":"BioHippo","version":"1.0","type":"link"}