{"product_id":"enzyfluo-human-mouse-rb-t821-826-phosphorylation-elisa-kit-bhe15600175","title":"EnzyFluo™ Human\/Mouse RB(T821\/826) Phosphorylation ELISA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e\n.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}\n.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}\n.bhp-desc p{margin:0 0 12px}\n.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}\n.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}\n.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}\n.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}\n\u003c\/style\u003e\n\u003ch2\u003eScientific Background\u003c\/h2\u003e\n\u003cp\u003eFor determination of RB(T821\/826) phosphorylation status in whole cells and evaluation of pathway modulation by activators, inhibitors, siRNA etc. Key Features: New and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs). Simple and convenient. Cells are directly cultured in 96-well plates. No cell lysis necessary. Accurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. Can be readily automated as a high-throughput 96-well plate assay for thousands.\u003c\/p\u003e\n\u003ch2\u003eAssay Principle\u003c\/h2\u003e\n\u003cp\u003eThis Cell-Based ELISA Kit measures EnzyFluo™ Human\/Mouse RB(T821\/826) Phosphorylation phosphorylation status directly in intact, fixed cells cultured in a 96-well plate — no cell lysis required. Cells are fixed, permeabilized, and incubated with a phospho-specific primary antibody followed by an HRP-conjugated secondary antibody. A fluorogenic substrate provides a signal proportional to phosphorylation level, normalized to total cellular protein measured in the same well to minimize well-to-well variation.\u003c\/p\u003e\n\u003ch2\u003eKey Features\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eNew and improved. Total assay time reduced from the standard 21 hours to 6.5 hours (hands-on time 2.5 hrs).\u003c\/li\u003e\n\u003cli\u003eSimple and convenient. Cells are directly cultured in 96-well plates. No cell lysis is necessary.\u003c\/li\u003e\n\u003cli\u003eAccurate and high-throughput. Protein phosphorylation is normalized to total cellular protein in the same well, greatly minimizing well-to-well variations. It can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003ePerformance Specifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\u003ctbody\u003e\n\u003ctr\u003e\n\u003cth\u003eTotal Assay Time\u003c\/th\u003e\n\u003ctd\u003e6.5 hours (hands-on: 2.5 hours)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eCompatible Sample Types\u003c\/th\u003e\n\u003ctd\u003eCells (whole cells in 96-well plate)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecies Reactivity\u003c\/th\u003e\n\u003ctd\u003eHuman, Rat, Mouse\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eDetection Method\u003c\/th\u003e\n\u003ctd\u003eFluorometric\/Colorimetric (FL530\/585 nm, 360\/450 nm)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eStorage\u003c\/th\u003e\n\u003ctd\u003e-20 °C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\u003c\/table\u003e\n\u003cp\u003e\u003cspan class=\"bhp-qc-badge\"\u003e✓ Research-Grade Validation\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\n\u003cstrong\u003eResearch Use Only (RUO).\u003c\/strong\u003e This product is intended for research purposes only and is not approved for diagnostic, therapeutic, or clinical use.\u003c\/div\u003e\n\u003cp\u003eHandle reagents in accordance with institutional biosafety guidelines. Refer to the Safety Data Sheet (SDS) for complete hazard and handling information. Contains components that may require special disposal procedures per local regulations.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests","offer_id":53337687916909,"sku":"ERBT-100","price":539.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ERBTfig.jpg?v=1778948053","url":"https:\/\/www.ebiohippo.com\/products\/enzyfluo-human-mouse-rb-t821-826-phosphorylation-elisa-kit-bhe15600175","provider":"BioHippo","version":"1.0","type":"link"}