{"product_id":"enzylight-cytotoxicity-assay-kit-bht15600141","title":"EnzyLight™ Cytotoxicity Assay Kit","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eFor rapid, bioluminescent luciferin\/luciferase based assay for cell viability, proliferation, cytotoxcity and high-throughput screen for anticancer agents. The assay uses Luminescence for signal readout. Compatible sample input includes Cells. Typical stated assay timing is 2 min.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003e\n\u003cstrong\u003eReadout format:\u003c\/strong\u003e Luminescence supports plate-based signal acquisition and consistent comparison across matched samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eSample compatibility:\u003c\/strong\u003e The stated sample scope includes Cells, which is useful when aligning matrix type with calibration and control design.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eAnalytical range context:\u003c\/strong\u003e The supplied specifications include a stated detection limit of 50 cells for interpreting low-signal samples.\u003c\/li\u003e\n  \u003cli\u003e\n\u003cstrong\u003eFeature emphasis:\u003c\/strong\u003e Safe. Non-radioactive assay (cf. 3H-thymidine incorporation assay).\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eAdditional feature notes highlight Sensitive and accurate. As low as 50 cells can be quantified; Homogeneous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Available format information for this listing includes 100 Tests.\u003c\/p\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThis product is centered on measurement of enzylight cytotoxicity within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.\u003c\/p\u003e\n\n\u003ch2\u003eMore details\u003c\/h2\u003e\n\u003cp\u003e\u003ci\u003e Adenosine 5’-triphosphate \u003c\/i\u003e(ATP) is the chemical energy for cellular metabolism and is often referred to as “energy currency” of the cell. ATP is produced only in living cells during photosynthesis and cellular respiration and consumed in cellular processes including biosynthetic reactions, motility and cell division. It is a key indicator of cellular activity and has been utilized as a measure of cell viability and cytotoxicity in research and drug discovery. BioAssay Systems’ EnzyLight™ Cytotoxicity Assay Kit provides a rapid method to measure intracellular ATP, cell viability and cytotoxicity. The single working reagent lyses cells to release ATP, which, in the presence of luciferase, immediately reacts with the Substrate D-luciferin to produce light. The light intensity is a direct measure of intracellular ATP concentration and hence number of living cells. This non-radioactive, homogeneous cell-based assay can be conveniently performed in microplates. The reagent is compatible with all culture media and liquid handling systems for high-throughput screening applications in 96-well and 384-well plates.\u003c\/p\u003e\n\n\u003ch2\u003eDetection method\u003c\/h2\u003e\n\u003cp\u003eLuminescence.\u003c\/p\u003e\n\n\u003ch2\u003eDetection limit and analytical sensitivity\u003c\/h2\u003e\n\u003cp\u003eReported detection limit: 50 cells.\u003c\/p\u003e\n\n\u003ch2\u003eProcedures and timing\u003c\/h2\u003e\n\u003cp\u003eStated procedure or timing information: 2 min.\u003c\/p\u003e\n\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003ePlate-based quantification and side-by-side group comparison remain central use cases for this assay format.\u003c\/li\u003e\n  \u003cli\u003eThe product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.\u003c\/li\u003e\n  \u003cli\u003eThe description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eQuantify cell viability or cytotoxicity in cultured cells by Luminescence readout.\u003c\/li\u003e\n  \u003cli\u003eCompare dose-response effects of compounds across treated cell-culture wells.\u003c\/li\u003e\n  \u003cli\u003eMonitor cytotoxicity time-courses after toxin, detergent, or drug exposure in culture.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eInterpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.\u003c\/p\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n  \u003cli\u003eMatrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.\u003c\/li\u003e\n  \u003cli\u003eUse appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003c!-- Sources (internal):\n- Product Description column\n- Key Features column\n- More Details column\n- Method \/ Sample Type(s) \/ Assay Time \/ Detection Limit \/ Detection Method columns\n- Procedures column\n- Screening Services column\n--\u003e","brand":"BioAssay Systems","offers":[{"title":"100 Tests","offer_id":53238314959213,"sku":"ECTX-100","price":349.17,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ECTXfig.jpg?v=1776668351","url":"https:\/\/www.ebiohippo.com\/products\/enzylight-cytotoxicity-assay-kit-bht15600141","provider":"BioHippo","version":"1.0","type":"link"}