{"product_id":"eps8-antibody-epidermal-growth-factor-receptor-pathway-substrate-8-bha17136271","title":"EPS8 Antibody \/ Epidermal growth factor receptor pathway substrate 8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eEPS8 Antibody \/ Epidermal growth factor receptor pathway substrate 8 is a anti-EPS8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm, plasma membrane.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e EPS8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, IP, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eEPS8 antibody detects Epidermal growth factor receptor pathway substrate 8, a signaling and cytoskeletal regulatory protein encoded by the EPS8 gene located on chromosome 12p12.3. EPS8 acts as a downstream effector of receptor tyrosine kinases, particularly the epidermal growth factor receptor (EGFR), and plays essential roles in actin remodeling, cell motility, and growth signaling. It is expressed in epithelial, neuronal, and fibroblast cells, where it integrates extracellular growth cues with cytoskeletal dynamics and membrane trafficking.\u003cbr\u003e\u003cbr\u003eStructurally, EPS8 contains an N-terminal phosphotyrosine-binding (PTB) domain, a central SH3 domain, and a C-terminal effector region responsible for actin bundling and regulation. It belongs to the EPS8 protein family, which includes EPS8L1-3, sharing conserved SH3 domains involved in receptor-proximal signaling. EPS8 interacts with SOS1, ABI1, and E3B1 (ABI2) to form a complex that links receptor activation to Rac-mediated actin remodeling. Known binding partners also include EGFR, SHC, and Grb2, which recruit EPS8 to the plasma membrane upon receptor stimulation.\u003cbr\u003e\u003cbr\u003eFunctionally, EPS8 regulates actin cytoskeleton organization, cell migration, and proliferation. It enhances Rac GTPase activation and promotes formation of membrane ruffles and lamellipodia during cell movement. EPS8 also acts as a scaffold that coordinates signaling between growth factor receptors and cytoskeletal regulators. In neurons, it supports dendritic spine development and synaptic plasticity. In epithelial tissues, it contributes to cell-cell adhesion and polarity maintenance. Known ligands and substrates include actin filaments and receptor tyrosine kinases such as EGFR and FGFR.\u003cbr\u003e\u003cbr\u003eEPS8 expression is dynamically regulated during development and in response to growth factor stimulation. It is highly expressed in embryonic tissues undergoing morphogenesis and wound repair. Dysregulation of EPS8 has been implicated in cancer, where overexpression enhances migration, invasion, and resistance to apoptosis. Elevated EPS8 correlates with aggressive behavior in colorectal, breast, and pancreatic cancers. Pathway involvement includes EGFR signaling, actin cytoskeleton organization, and Ras\/Rac-mediated signaling networks.\u003cbr\u003e\u003cbr\u003eThe EPS8 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=EPS8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=EPS8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=EPS8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/EPS8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=EPS8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323984237,"sku":"FY13369","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_706b5ad3-dca4-4a69-a8ee-9ab6882b7cdb.jpg?v=1782237113","url":"https:\/\/www.ebiohippo.com\/products\/eps8-antibody-epidermal-growth-factor-receptor-pathway-substrate-8-bha17136271","provider":"BioHippo","version":"1.0","type":"link"}