{"product_id":"er-alpha-antibody-estrogen-receptor-alpha-bha17132958","title":"ER alpha Antibody \/ Estrogen Receptor alpha","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eER alpha Antibody \/ Estrogen Receptor alpha is an antibody targeting \u003cstrong\u003eER\u003c\/strong\u003e. Host: \u003cstrong\u003eMouse\u003c\/strong\u003e. Monoclonal. Clone: \u003cstrong\u003eAER311\u003c\/strong\u003e. Format: Purified. Applications: FACS, IF.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ER.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Estrogen Receptor purified from cow uterus was used as the immunogen for the ER alpha antibody. The epitope of this mAb….\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Human.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eLocalization:\u003c\/strong\u003e Nuclear.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate:\u003c\/strong\u003e Unconjugated (no label listed).\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background (from provided description)\u003c\/h2\u003e\u003cp\u003eThis mAb is specific to ER alpha and shows minimal cross-reaction with other members of the family. Epitope of this mAb is mapped between amino acids 495-595 of E\/F domain. Estrogen Receptor is an important regulator of growth and differentiation in the mammary gland. Presence of Estrogen Receptor in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eIF\/ICC:\u003c\/strong\u003e subcellular localization imaging with proper background controls.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eAccount for isoforms, paralogs, and post-translational modifications when interpreting signal.\u003c\/li\u003e\n\u003cli\u003eSupport specificity with biological controls (e.g., KO\/KD where available) and technical controls (e.g., secondary-only\/isotype).\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt Knowledgebase (UniProt) – https:\/\/www.uniprot.org\/ - NCBI Gene (NIH\/NLM) – https:\/\/www.ncbi.nlm.nih.gov\/gene\/ - ZFIN – https:\/\/zfin.org\/ - Ensembl – https:\/\/www.ensembl.org\/ - Nature Methods (2015): A proposal for validation of antibodies – https:\/\/www.nature.com\/articles\/nmeth.3230 --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.2 mg\/ml in 1X PBS with 0.1 mg\/ml BSA (US sourced), 0.05% sodium azide \/ 100 ug","offer_id":53046676947309,"sku":"V5187-100UG","price":559.0,"currency_code":"USD","in_stock":true},{"title":"0.2 mg\/ml in 1X PBS with 0.1 mg\/ml BSA (US sourced), 0.05% sodium azide \/ 20 ug","offer_id":53046821486957,"sku":"V5187-20UG","price":259.0,"currency_code":"USD","in_stock":true},{"title":"1 mg\/ml in 1X PBS; BSA free, sodium azide free \/ 100 ug","offer_id":53046821519725,"sku":"V5187SAF-100UG","price":559.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_388d11da-28b0-4f30-8c66-934f8d6cf585.jpg?v=1782236960","url":"https:\/\/www.ebiohippo.com\/products\/er-alpha-antibody-estrogen-receptor-alpha-bha17132958","provider":"BioHippo","version":"1.0","type":"link"}