{"product_id":"er-expressing-epithelial-endometrial-cancer-cell-line-hci-ec-23-bhc10902113","title":"ER Expressing Epithelial Endometrial Cancer Cell Line (HCI-EC-23)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThe HCI-EC-23 cell line is a novel, adherent endometrioid endometrial cancer (EEC) model that was developed from a patient-derived xenograft (PDX) model. The primary tumor was microsatellite instability-high (MSI-high) and estrogen receptor (ER) positive.\u003c\/p\u003e\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eModel identity:\u003c\/strong\u003e ER Expressing Epithelial Endometrial Cancer Cell Line (HCI-EC-23) is supplied as a tumor cell line derived from Human endometrium.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth properties:\u003c\/strong\u003e Adherent, epithelial\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eProduct format:\u003c\/strong\u003e Frozen, BSL-2\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eThis cell-based model is generally used in endometrium biology, phenotype comparison, and assay development studies. Donor\/background information is available for contextual interpretation.\u003c\/p\u003e\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003eThe cell line retains these characteristics, maintaining ER expression and estrogen responsiveness both in vitro and in vivo. Characterization of HCI-EC-23 revealed that it is mutated in many commonly altered genes in EEC, such as PTEN, PIK3CA, and ARID1A, and has relatively few copy-number alterations. It exhibits paradoxical activation of ER by tamoxifen and shows an anti-proliferative response to progesterone, which is crucial for translational studies involving synthetic progesterone treatments. In vitro, HCI-EC-23 demonstrates a strong estrogen dependence for proliferation, with estrogen significantly boosting growth and progesterone reducing it. HCI-EC-23 tumors in mice also show estrogen dependence, with a reduction in tumor volume when treated with progesterone. The cell line’s unique features, including its MSI-high status, ER positivity, and distinct genetic background compared to the Ishikawa cell line, make it a valuable model for studying the hormonal underpinnings of endometrial cancer. HCI-EC-23 is well-suited for genetic screens, drug testing, and understanding the molecular aspects of hormonally driven endometrial cancer. Donor\/background information provided for this product: Female, 66, Grade 2, Stage 1A endometrioid endometrial carcinoma.\u003c\/p\u003e\n\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCell-line models continue to be used for tumor phenotype comparison, pathway perturbation studies, and assay development in controlled in vitro systems.\u003c\/li\u003e\n\u003cli\u003eEngineered and subtype-defined tumor lines are often used to compare growth behavior, reporter output, and response patterns across matched experimental conditions.\u003c\/li\u003e\n\u003cli\u003eWhen metastatic or lineage features are described, investigators commonly interpret results alongside morphology, passage history, and culture environment.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eCancer biology studies that compare proliferation-associated behavior, morphology, and pathway responses in vitro.\u003c\/li\u003e\n\u003cli\u003eAssay development for treatment response, reporter monitoring, or phenotype comparison under matched culture conditions.\u003c\/li\u003e\n\u003cli\u003eSide-by-side comparison of engineered versus parental background characteristics when relevant to the study design.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp\u003eChanges in morphology, growth rate, viability, or reporter signal are typically interpreted together with passage history, culture matrix, and the specified growth conditions for the model.\u003c\/p\u003e\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMorphology, doubling behavior, and reporter or marker output can shift with passage number, substrate choice, and medium composition; these variables should be recorded alongside experimental readouts.\u003c\/li\u003e\n\u003cli\u003eMatched controls such as parental cells, untreated cultures, or parallel cultures maintained under identical conditions help distinguish background effects from biology of interest.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eCulture and product details\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eGrowth Conditions:\u003c\/strong\u003e PriCoat™ T25 Flasks (G299) or Applied Cell Extracellular Matrix (G422) are recommended for optimal cell culture. RPMI 1640 Medium (TM503) + 10% FBS(Regular*) + 1% Penicillin\/Streptomycin Solution (G255), 37.0°C, 5% CO₂. *Do not heat-inactivate\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSeeding Density (cells\/cm²):\u003c\/strong\u003e 30,000\u003c\/li\u003e\n\u003c\/ul\u003e","brand":"Applied Biological Materials (abm) Inc.","offers":[{"title":"1x10\u003csup\u003e6\u003c\/sup\u003e cells \/ 1.0 ml","offer_id":53180541698413,"sku":"T8158","price":970.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/exmaiaAZZjNTUKP4PtYWmZpYWV0urFNOEoVvyWYG.png?v=1774957856","url":"https:\/\/www.ebiohippo.com\/products\/er-expressing-epithelial-endometrial-cancer-cell-line-hci-ec-23-bhc10902113","provider":"BioHippo","version":"1.0","type":"link"}