{"product_id":"ercc4-antibody-biotin-conjugated-bha10528075","title":"ERCC4 Antibody, Biotin conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThis polyclonal anti-ERCC4 antibody raised in Rabbit, conjugated with Biotin, tested in ELISA. Designed to detect ERCC4 in Human for epigenetics and nuclear signaling research.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human DNA repair endonuclease XPF protein (656-753AA) — determines epitope; confirm sample prep compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary matching isotype IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Multiple epitopes = robust signal; lot-to-lot variability — include controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Match secondary, Protein A\/G, and isotype controls to IgG.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Enriches Ig classes; reduces non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eBiotin:\u003c\/strong\u003e Streptavidin\/avidin amplification; block endogenous biotin in biotin-rich samples.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003e\u003cstrong\u003eERCC4\u003c\/strong\u003e (also: DNA excision repair protein ERCC 4, DNA excision repair protein ERCC-4, DNA excision repair protein ERCC4, DNA repair endonuclease XPF, DNA repair protein complementing XP F cells, DNA repair protein complementing XP-F cells, ERCC 11, ERCC 4, ERCC11, ERCC4, Excision repair complementing defective in Chinese hamster, Excision repair cross complementing rodent repair deficiency complementation group 4, excision repair cross-complementation group 4, FANCQ, RAD 1, RAD1, Xeroderma pigmentosum complementation group F, Xeroderma pigmentosum group F complementing protein, Xeroderma pigmentosum group F-complementing protein, Xeroderma pigmentosum VI, XP, group G, XP6, XPF_HUMAN) — studied in Human systems. Expression, localization, and PTMs vary across cell types and disease states. Consult UniProt, NCBI Gene, and primary literature for current ERCC4 annotation relevant to epigenetics and nuclear signaling.\u003c\/p\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Quantify soluble target; ensure samples within the linear detection range.\u003c\/li\u003e\u003c\/ul\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e IgG from Rabbit at equivalent concentration.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity:\u003c\/strong\u003e Polyclonal: validate with KO\/KD controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Verify dilution linearity in your sample matrix.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eReactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation requires validation.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\u003c!-- Sources: UniProt, NCBI Gene, Cusabio, Human Protein Atlas, PubMed --\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53240812306797,"sku":"CSB-PA849795HD01HU-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53240913985901,"sku":"CSB-PA849795HD01HU-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/Cusabio_Antibody_Placeholder_89550aea-b223-44e1-87d5-457df751c249.jpg?v=1784926873","url":"https:\/\/www.ebiohippo.com\/products\/ercc4-antibody-biotin-conjugated-bha10528075","provider":"BioHippo","version":"1.0","type":"link"}