{"product_id":"ercc5-antibody-excision-repair-cross-complementation-group-5-bha17135392","title":"ERCC5 Antibody \/ Excision repair cross-complementation group 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eERCC5 Antibody \/ Excision repair cross-complementation group 5 is a anti-ERCC5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ERCC5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, IF, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eERCC5 antibody detects Excision repair cross-complementation group 5 protein, a structure-specific endonuclease essential for nucleotide excision repair (NER). Also known as Xeroderma pigmentosum group G protein (XPG), ERCC5 catalyzes 3' incision at sites of DNA damage, removing bulky lesions such as ultraviolet-induced pyrimidine dimers. The ERCC5 antibody is widely used in DNA repair, cancer, and dermatological research to study genome stability, DNA damage responses, and repair-deficient disorders.\u003cbr\u003e\u003cbr\u003eERCC5 is encoded by the ERCC5 gene located on human chromosome 13q33.1. The protein is approximately 118 kilodaltons in size and belongs to the XPG\/RAD2 family of structure-specific nucleases. It contains conserved N- and I-domains forming the catalytic core, as well as C-terminal regions mediating interactions with transcription and repair factors such as TFIIH and PCNA. Through these interactions, ERCC5 coordinates incision with repair synthesis during the NER process.\u003cbr\u003e\u003cbr\u003eThe ERCC5 antibody typically detects a strong nuclear band near 133 kilodaltons by western blot. Immunofluorescence reveals nuclear localization, often enriched at DNA damage foci following UV or oxidative stress exposure. Functional studies demonstrate that ERCC5 excises damaged DNA downstream of the lesion, allowing DNA polymerases to resynthesize the correct strand. Mutations in ERCC5 cause Xeroderma pigmentosum complementation group G (XP-G) and Cockayne syndrome, conditions characterized by UV hypersensitivity, neurological abnormalities, and premature aging.\u003cbr\u003e\u003cbr\u003eBeyond its canonical repair function, ERCC5 also contributes to transcription-coupled repair and RNA polymerase II-mediated gene expression. It participates in DNA replication stress responses and protects replication forks from collapse under genotoxic stress. Dysregulation or reduced expression of ERCC5 has been linked to increased cancer susceptibility, particularly skin, liver, and lung cancers.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ERCC5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ERCC5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ERCC5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ERCC5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ERCC5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047289971053,"sku":"FY12489","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_729840b0-9778-4b55-a3ea-66d7b33e6b18.jpg?v=1782237015","url":"https:\/\/www.ebiohippo.com\/products\/ercc5-antibody-excision-repair-cross-complementation-group-5-bha17135392","provider":"BioHippo","version":"1.0","type":"link"}