{"product_id":"ere-reporter-lentivirus-bhv19400042","title":"ERE Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe estrogen response element (ERE) is a DNA sequence that mediates transcription driven by the estrogen receptor (ER), a ligand-activated nuclear receptor encoded by ESR1. Upon binding estrogens such as estradiol, ER dimerizes, translocates to the nucleus, and binds estrogen response elements to regulate genes controlling reproductive tissue development, bone density, and cardiovascular and metabolic physiology. ER signaling is a central driver of hormone-responsive breast cancer, where it remains a major therapeutic target. ERE-driven reporters give a quantitative readout of estrogen receptor transcriptional activity for endocrinology, steroid hormone, and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe ERE Reporter Lentivirus is a transcription factor reporter system that places a reporter gene under the control of tandem estrogen response elements coupled to a minimal promoter, giving a sensitive fluorescent or luminescent readout of estrogen receptor activation. Reporter options include EGFP, GFP, mCherry, RFP, firefly luciferase, and Renilla luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eApplications include monitoring ER signaling pathway activity, characterizing estrogens and anti-estrogens, and screening endocrine-active compounds in endocrinology and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a EGFP, Firefly Luc, mCherry, Renilla Luc, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the Estrogen receptor (ER) signaling pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616506221,"sku":"LTV-0046-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310896701805,"sku":"LTV-0046-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310896734573,"sku":"LTV-0046-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896767341,"sku":"LTV-0046-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310896800109,"sku":"LTV-0046-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310896832877,"sku":"LTV-0046-6S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Renilla Luc \/ Blasticidin \/ 2x10^6","offer_id":53310896865645,"sku":"LTV-0046-4SIC","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/ERE-TAG-PURO-2.jpg?v=1776934393","url":"https:\/\/www.ebiohippo.com\/products\/ere-reporter-lentivirus-bhv19400042","provider":"BioHippo","version":"1.0","type":"link"}