{"product_id":"erp27-antibody-endoplasmic-reticulum-resident-protein-27-txndc13-bha17135982","title":"ERP27 Antibody \/ Endoplasmic reticulum resident protein 27 \/ TXNDC13","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eERP27 Antibody \/ Endoplasmic reticulum resident protein 27 \/ TXNDC13 is a anti-ERP27 Rabbit antibody Recombinant Rabbit Monoclonal clone 32E24 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ERP27\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32E24, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eERP27 antibody detects Endoplasmic reticulum resident protein 27, also called TXNDC13, encoded by the ERP27 gene. This protein belongs to the protein disulfide isomerase (PDI) family but is unique because it lacks catalytic cysteine residues required for disulfide bond formation. Instead, ERP27 functions as a non catalytic PDI family member that likely regulates protein folding through binding to unfolded substrates. ERP27 antibody provides a useful tool for studying protein folding, endoplasmic reticulum stress responses, and secretory pathway homeostasis.\u003cbr\u003e\u003cbr\u003eEndoplasmic reticulum resident protein 27 is localized to the lumen of the endoplasmic reticulum, where it interacts with nascent polypeptides and folding intermediates. Although it lacks enzymatic activity, ERP27 binds to unfolded proteins, potentially presenting them to catalytic PDIs for isomerization. Research using ERP27 antibody has demonstrated that it contributes to quality control of protein folding, ensuring only properly folded proteins proceed through the secretory pathway. By acting as a chaperone like molecule, ERP27 complements enzymatic PDIs in maintaining proteostasis.\u003cbr\u003e\u003cbr\u003eERP27 is upregulated during conditions of endoplasmic reticulum stress, such as accumulation of unfolded proteins or perturbations in calcium homeostasis. Studies with ERP27 antibody have shown that its expression is regulated by the unfolded protein response, linking it to pathways that restore folding capacity and reduce stress. Dysregulation of ERP27 may contribute to diseases characterized by endoplasmic reticulum stress, including diabetes, neurodegeneration, and cancer. Understanding ERP27 expression and function helps clarify how cells cope with misfolded proteins and maintain secretory pathway fidelity.\u003cbr\u003e\u003cbr\u003eIn cancer, altered expression of endoplasmic reticulum chaperones influences tumor survival and progression. Research with ERP27 antibody has indicated that increased expression may provide tumor cells with resilience against proteotoxic stress, while reduced levels may impair secretory function. Its role in regulating folding efficiency positions ERP27 as an emerging factor in tumor biology and therapeutic resistance. Beyond cancer, ERP27 contributes to cellular stress adaptation across a wide range of tissues, including secretory cells of the pancreas and liver.\u003cbr\u003e\u003cbr\u003eERP27 antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting demonstrates induction under stress conditions, immunohistochemistry shows distribution in secretory tissues, and immunofluorescence confirms localization to the endoplasmic reticulum. These applications make ERP27 antibody valuable for research into protein folding, stress responses, and secretory pathway regulation.\u003cbr\u003e\u003cbr\u003eBy providing validated ERP27 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ERP27 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ERP27 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ERP27 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ERP27 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ERP27+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047311368557,"sku":"FY13080","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_a89227b7-4dee-44df-8591-42624af37fef.jpg?v=1772019387","url":"https:\/\/www.ebiohippo.com\/products\/erp27-antibody-endoplasmic-reticulum-resident-protein-27-txndc13-bha17135982","provider":"BioHippo","version":"1.0","type":"link"}