{"product_id":"espn-antibody-espin-bha17135543","title":"ESPN Antibody \/ Espin","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eESPN Antibody \/ Espin is a anti-ESPN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ESPN\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eESPN antibody detects Espin, an actin-binding protein essential for the assembly and maintenance of parallel actin bundles in sensory cells, particularly in the stereocilia of hair cells in the inner ear. ESPN is required for hearing and balance by supporting the structural integrity of mechanosensory projections. The ESPN antibody is widely used in auditory biology, cytoskeletal, and sensory neuroscience research to study actin organization, hair cell morphology, and mechanotransduction.\u003cbr\u003e\u003cbr\u003eESPN is encoded by the ESPN gene located on human chromosome 1p36.31. The protein exists in multiple isoforms ranging from 1,000 to 1,100 amino acids, each containing actin-binding and proline-rich domains that mediate crosslinking of actin filaments. ESPN localizes to microvillar and stereociliary actin cores, where it provides structural reinforcement and length regulation.\u003cbr\u003e\u003cbr\u003eThe ESPN antibody detects bands between 90 and 110 kilodaltons depending on isoform and shows intense labeling of stereocilia and microvilli under immunofluorescence microscopy. ESPN promotes actin bundle stability by counteracting depolymerizing factors such as cofilin, ensuring the long-term maintenance of hair cell stereocilia. Its overexpression induces elongation of actin protrusions, while loss of function causes disorganization and degeneration of stereocilia, leading to deafness.\u003cbr\u003e\u003cbr\u003eMutations in ESPN are responsible for autosomal recessive deafness and vestibular dysfunction in both humans and animal models. Beyond auditory tissue, ESPN contributes to brush border maintenance in epithelial cells and to filopodia dynamics in neurons and immune cells, reflecting its broad cytoskeletal role.\u003cbr\u003e\u003cbr\u003eBecause of its crucial function in actin filament assembly and mechanosensory integrity, ESPN serves as a vital biomarker for studies of hearing, balance, and cytoskeletal structure.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ESPN - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ESPN - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ESPN - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ESPN - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ESPN+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047295279469,"sku":"FY12640","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d90c6867-eb2c-4e5e-a8df-409ef164e37b.jpg?v=1782237020","url":"https:\/\/www.ebiohippo.com\/products\/espn-antibody-espin-bha17135543","provider":"BioHippo","version":"1.0","type":"link"}