{"product_id":"etv2-antibody-ets-variant-transcription-factor-2-bha17136236","title":"ETV2 Antibody \/ ETS variant transcription factor 2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eETV2 Antibody \/ ETS variant transcription factor 2 is a anti-ETV2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e ETV2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eETV2 antibody detects ETS variant transcription factor 2, a nuclear DNA-binding protein encoded by the ETV2 gene on chromosome 19p13.3. ETV2 belongs to the ETS family of transcription factors and is an essential regulator of vascular and hematopoietic development. It is highly expressed in endothelial progenitor cells, embryonic mesoderm, and early hematopoietic lineages, where it drives the transcription of genes required for blood vessel and blood cell formation. ETV2 functions as a master transcriptional activator that initiates endothelial lineage commitment during embryogenesis.\u003cbr\u003e\u003cbr\u003eETV2 directly binds to ETS consensus sequences in the promoters of endothelial genes, activating transcription of critical targets such as VEGFR2 (KDR), TIE2, and CDH5 (VE-cadherin). This activity positions ETV2 at the top of the transcriptional hierarchy controlling vasculogenesis and angiogenesis. It cooperates with transcription factors such as GATA2 and FOXC2 to orchestrate endothelial differentiation and vascular remodeling. ETV2 also transiently regulates hematopoietic gene expression, linking early vascular and blood development.\u003cbr\u003e\u003cbr\u003eStructurally, ETV2 contains an ETS DNA-binding domain responsible for recognizing GGAA\/T motifs in target promoters and a transactivation domain that recruits coactivators such as CBP\/p300. These interactions enable the chromatin remodeling necessary for endothelial gene induction. ETV2 belongs to the ETS family of transcription factors, which share highly conserved DNA-binding domains that control diverse developmental and cellular processes.\u003cbr\u003e\u003cbr\u003eFunctionally, ETV2 plays a crucial role in embryonic vasculogenesis, angiogenesis, and reprogramming of somatic cells toward endothelial fates. Forced expression of ETV2 in fibroblasts can induce endothelial gene expression, demonstrating its potency as a lineage reprogramming factor. ETV2 also participates in VEGF and MAPK signaling pathways, coordinating growth factor responses during vascular morphogenesis. Co-localization studies have shown ETV2 associating with GATA2 and TAL1 in the nucleus, forming transcriptional complexes essential for endothelial specification.\u003cbr\u003e\u003cbr\u003eDysregulation of ETV2 expression disrupts vascular and hematopoietic development and has been implicated in congenital heart defects and embryonic lethality in knockout models. In adult tissues, ETV2 expression is normally repressed, but its reactivation can promote angiogenesis in ischemic and regenerative contexts. Pathway involvement includes VEGF signaling, Notch signaling, and angiogenic transcriptional networks that coordinate vessel growth and stabilization.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using ETV2 antibody reveals strong nuclear localization in vascular endothelial cells and embryonic tissues. ETV2 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=ETV2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=ETV2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=ETV2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/ETV2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=ETV2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047323099501,"sku":"FY13334","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_0b8edaac-a595-419d-aa28-09f2f807e02a.jpg?v=1782237105","url":"https:\/\/www.ebiohippo.com\/products\/etv2-antibody-ets-variant-transcription-factor-2-bha17136236","provider":"BioHippo","version":"1.0","type":"link"}