{"product_id":"fast-magbead-tissue-dna-kit-bht15200022","title":"Fast Magbead Tissue DNA Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis kit provides a simple, rapid and efficient method for extracting DNA from fresh or frozen tissues. After tissue lysis, the DNA binds to the surface of silica-coated magnetic beads. After rinsing, the high-purity DNA is eluted in buffer TB or deionized water. The purified DNA can be directly used in PCR, Real-time PCR, SNP, STR, next-generation sequencing, pharmacogenomics research and so on. Besides being able to rapidly extract DNA from animal tissues, this kit is also applicable to the extraction of DNA from samples such as blood, cells and bacteria.\u003c\/p\u003e\n\u003cp\u003eThe protocol processes 25–30 mg of tissue, with less than 10 mg used for spleen, or 200 µL of blood. Tissue is lysed with Buffer ATL and Proteinase K at 56°C for 30 min, treated with RNase A for 10 min, then bound with Buffer AL, isopropanol and Mag beads PN. The beads are washed with Buffer MB, Buffer DW and twice with 75% ethanol at 1200 rpm for 3 min each, dried for 3–5 min and eluted with 80–100 µL of Buffer TB at 56°C for 10 min. The A260\/A280 ratio of pure DNA is 1.7–1.9.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSilica-coated magnetic beads (Mag beads PN)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample types\u003c\/th\u003e\n\u003ctd\u003eFresh or frozen tissue, blood, cells and bacteria\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input (tissue)\u003c\/th\u003e\n\u003ctd\u003e25–30 mg; less than 10 mg for spleen\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input (blood)\u003c\/th\u003e\n\u003ctd\u003e200 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity (A260\/A280)\u003c\/th\u003e\n\u003ctd\u003e1.7–1.9 for pure DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e80–100 µL Buffer TB at 56°C for 10 min\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eEnzymes supplied\u003c\/th\u003e\n\u003ctd\u003eProteinase K (20 mg\/mL), RNase A (100 mg\/mL)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e96 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer ATL\u003c\/td\u003e\n\u003ctd\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer AL\u003c\/td\u003e\n\u003ctd\u003e36 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer MB\u003c\/td\u003e\n\u003ctd\u003e70 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer DW\u003c\/td\u003e\n\u003ctd\u003e70 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer TB\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteinase K (20 mg\/mL)\u003c\/td\u003e\n\u003ctd\u003e2 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase A (100 mg\/mL)\u003c\/td\u003e\n\u003ctd\u003e0.6 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eMag beads PN\u003c\/td\u003e\n\u003ctd\u003e4 × 1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDNA extraction from fresh or frozen animal tissue\u003c\/li\u003e\n\u003cli\u003eDNA extraction from blood, cells and bacteria\u003c\/li\u003e\n\u003cli\u003ePCR and Real-time PCR\u003c\/li\u003e\n\u003cli\u003eSNP and STR analysis\u003c\/li\u003e\n\u003cli\u003eNext-generation sequencing and pharmacogenomics research\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eAll components can be stably stored in a dry environment at room temperature (15–30°C), with a shelf life of 1 year.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eMag beads PN must not be frozen or subjected to high-speed centrifugation.\u003c\/li\u003e\n\u003cli\u003eRedissolve buffers if crystals form before use.\u003c\/li\u003e\n\u003cli\u003eGram-positive bacteria require lysozyme pretreatment.\u003c\/li\u003e\n\u003cli\u003eUse less than 10 mg of sample for spleen tissue.\u003c\/li\u003e\n\u003cli\u003eThe 75% ethanol wash is not listed among the kit components and must be prepared separately.\u003c\/li\u003e\n\u003cli\u003eIsopropanol is required at the binding step and is not supplied.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP026\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200022\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"96 T","offer_id":53965512311149,"sku":"EP026-96T","price":442.0,"currency_code":"USD","in_stock":true},{"title":"96 T x 10","offer_id":53965516865901,"sku":"EP026-96TX10","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP026-a-1.jpg?v=1789419115","url":"https:\/\/www.ebiohippo.com\/products\/fast-magbead-tissue-dna-kit-bht15200022","provider":"BioHippo","version":"1.0","type":"link"}