{"product_id":"fat1-antibody-drosophila-melanogaster-bha17116960","title":"FAT1 Antibody (Drosophila melanogaster)","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eThe FAT proteins are members of the Cadherin superfamily homologous to the Drosophila Fat protein that functions as a positive regulator of planar cell polarity in the Drosophila wing. FAT1 is an unusual cadherin that controls cell growth and planar polarity while acting as a tumor suppressor. FAT1 is a proximal element of a signaling pathway that determines both cellular polarity in the plane of the monolayer and directed actin-dependent cell motility. FAT1 is localized at the leading edge of lamellipodia, filopodia and microspike tips where it directly interacts with Ena\/VASP proteins to regulate the actin polymerization complex. When targeted to mitochondrial outer leaflets, the cytoplasmic domain of FAT1 recruits components of the actin polymerization machinery sufficient to induce ectopic actin polymerization. FAT1 expression in vascular smooth muscle cells (VSMCs) increases significantly after arterial injury or growth factor stimulation, implicating FAT1 in the control of VSMC functions central to vascular remodeling by facilitating migration and limiting proliferation. FAT1 is also involved in psychic disorders, and its action may be of patho-physiological importance.\u003c\/p\u003e\u003cp\u003eThis anti-FAT1 antibody is supplied as Purified (Mouse, Monoclonal (mouse origin), clone FAT1-3D7\/1, Mouse IgM, kappa, Unconjugated) and is designed to support common target-detection workflows after the on-page specifications.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e FAT1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Purified\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Drosophila melanogaster\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (listed):\u003c\/strong\u003e ELISA, WB, IF\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eConjugate:\u003c\/strong\u003e Unconjugated\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eClone and antibody class:\u003c\/strong\u003e Monoclonal (mouse origin), clone FAT1-3D7\/1, Mouse IgM, kappa\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eBecause antibody performance can depend on epitope context, sample preparation, and biological state, interpret signals using appropriate controls and orthogonal evidence when possible.\u003c\/p\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cp\u003eFAT1 is referenced in public gene\/protein resources (e.g., UniProt and NCBI Gene), which provide curated names\/synonyms, protein features, and pathway context. When designing assays, consider potential isoforms, post-translational modifications, and cell-type specific expression that may influence observed signal.\u003c\/p\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eProfiling FAT1 expression across model systems, perturbations, and time points to support mechanistic hypotheses.\u003c\/li\u003e\n\u003cli\u003eCombining antibody-based detection with multi-omics or imaging readouts to link FAT1 signal with phenotype.\u003c\/li\u003e\n\u003cli\u003eUsing well-matched controls (isotype controls, genetic perturbations, or independent reagents) to strengthen interpretation of target-associated signal.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eELISA\u003c\/li\u003e\n\u003cli\u003eWB\u003c\/li\u003e\n\u003cli\u003eIF\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eUse the listed applications as a starting point and tailor experimental design to your sample type and readout requirements.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecificity considerations:\u003c\/strong\u003e closely related family members, isoforms, or PTMs can affect apparent specificity; confirm with independent approaches when critical.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eControls:\u003c\/strong\u003e include negative controls and, when feasible, genetic or pharmacologic perturbations to support target attribution in your system.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies and sample context:\u003c\/strong\u003e differences in sequence, expression, fixation, or extraction conditions can change signal behavior across models.\u003c\/li\u003e\n\u003c\/ul\u003e\u003c!-- Sources (internal): - UniProt Knowledgebase — UniProt — https:\/\/www.uniprot.org\/ - NCBI Gene — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/ - Ensembl Genome Browser — EMBL-EBI — https:\/\/www.ensembl.org\/ - Human Protein Atlas — SciLifeLab\/KTH — https:\/\/www.proteinatlas.org\/ - Gene Ontology — GO Consortium — https:\/\/geneontology.org\/ - Reactome Pathway Database — Reactome — https:\/\/reactome.org\/ - PubMed — NCBI — https:\/\/pubmed.ncbi.nlm.nih.gov\/ --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"0.2 mg\/ml in 1X PBS with 0.1 mg\/ml BSA (US sourced) and 0.05% sodium azide \/ 100 ug","offer_id":53045185446253,"sku":"V7767-100UG","price":559.0,"currency_code":"USD","in_stock":true},{"title":"0.2 mg\/ml in 1X PBS with 0.1 mg\/ml BSA (US sourced) and 0.05% sodium azide \/ 20 ug","offer_id":53045360918893,"sku":"V7767-20UG","price":259.0,"currency_code":"USD","in_stock":true},{"title":"1 mg\/ml in 1X PBS; BSA free, sodium azide free \/ 100 ug","offer_id":53045360951661,"sku":"V7767SAF-100UG","price":559.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_e3137a09-8734-448c-9212-a0711136932f.jpg?v=1771953193","url":"https:\/\/www.ebiohippo.com\/products\/fat1-antibody-drosophila-melanogaster-bha17116960","provider":"BioHippo","version":"1.0","type":"link"}