{"product_id":"fbxw8-antibody-f-box-wd-repeat-containing-protein-8-bha17135899","title":"FBXW8 Antibody \/ F-box\/WD repeat-containing protein 8","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eFBXW8 Antibody \/ F-box\/WD repeat-containing protein 8 is a anti-FBXW8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Human, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e FBXW8\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eFBXW8 antibody detects F-box\/WD repeat-containing protein 8, a substrate-recognition component of the SCF (SKP1-CUL1-F-box) E3 ubiquitin ligase complex. The UniProt recommended name is F-box\/WD repeat-containing protein 8 (FBXW8). This protein mediates selective ubiquitination and degradation of regulatory proteins that control cell cycle progression, growth, and signal transduction.\u003cbr\u003e\u003cbr\u003eFunctionally, FBXW8 antibody identifies a 616-amino-acid protein that contains an N-terminal F-box motif and multiple C-terminal WD repeats. The F-box domain binds to the SKP1 adaptor protein, anchoring FBXW8 within the SCF complex, while the WD repeats mediate substrate recognition. FBXW8 acts as a specificity factor that determines which proteins are targeted for ubiquitin-dependent degradation. Key substrates include IRS1, TSC2, and cyclin D1, linking FBXW8 to insulin signaling and cell cycle regulation.\u003cbr\u003e\u003cbr\u003eThe FBXW8 gene is located on chromosome 12q24.31 and encodes a cytoplasmic and nuclear protein expressed in most tissues, particularly in skeletal muscle and liver. FBXW8 associates with the cullin family protein CUL7 to form the CUL7-FBXW8 E3 ligase complex, distinct from classical SCF ligases. This complex regulates growth factor signaling and metabolic homeostasis by mediating the ubiquitination of signaling intermediates involved in mTOR and PI3K pathways.\u003cbr\u003e\u003cbr\u003eIn physiology, FBXW8 controls insulin receptor signaling by targeting IRS1 for degradation, preventing excessive insulin pathway activation. It also influences neuronal development and differentiation through ubiquitin-mediated turnover of growth-regulating proteins. Disruption of FBXW8 function leads to aberrant signaling, impaired growth, and metabolic imbalance. In cancer, altered FBXW8 expression contributes to tumor cell proliferation and metastasis by deregulating cyclin and kinase levels.\u003cbr\u003e\u003cbr\u003eFBXW8 antibody is widely used in cell cycle, signal transduction, and ubiquitin pathway research. It is suitable for immunoblotting, immunoprecipitation, and proteomic studies to identify protein interactions and degradation targets. This antibody provides a valuable tool for investigating the role of F-box proteins in proteostasis and oncogenic signaling. In metabolic studies, FBXW8 detection helps assess the regulation of insulin signaling components.\u003cbr\u003e\u003cbr\u003eStructurally, FBXW8's WD-repeat domain forms a beta-propeller structure that enables substrate recognition, while its F-box motif mediates SCF complex assembly. FBXW8 activity is modulated by phosphorylation, ubiquitination, and association with CUL7.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=FBXW8 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FBXW8 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=FBXW8 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/FBXW8 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=FBXW8+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047308812653,"sku":"FY12997","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_9b10dc6b-5854-4ea6-8782-1ee0d70dab1a.jpg?v=1782237074","url":"https:\/\/www.ebiohippo.com\/products\/fbxw8-antibody-f-box-wd-repeat-containing-protein-8-bha17135899","provider":"BioHippo","version":"1.0","type":"link"}