{"product_id":"fcer2-antibody-hrp-conjugated-bha10505422","title":"FCER2 Antibody, HRP conjugated","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\n\u003cp\u003eThis is a polyclonal anti-FCER2 antibody raised in Rabbit, conjugated with HRP, with confirmed utility in ELISA. Designed to detect FCER2 in Human, supporting researchers in immunology contexts.\u003c\/p\u003e\n\n\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunogen:\u003c\/strong\u003e Recombinant Human Low affinity immunoglobulin epsilon Fc receptor protein (48-321AA) — determines the epitope region recognized; confirm epitope compatibility with your sample preparation and expected PTMs.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHost (Rabbit):\u003c\/strong\u003e Requires anti-rabbit-IgG secondary reagents. Mismatched secondaries result in no signal. Consider host species when multiplexing.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePolyclonal:\u003c\/strong\u003e Recognizes multiple epitopes — robust signal across varied preparations, with inherent lot-to-lot variability. Isotype controls and lot qualification recommended.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype (IgG):\u003c\/strong\u003e Secondary antibodies and isotype controls must match IgG from Rabbit. Relevant for multiplexing and Protein A\/G compatibility.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003ePurification (Protein G purification):\u003c\/strong\u003e Reduces non-specific immunoglobulin populations, improving signal-to-noise in quantitative immunoassay formats.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eHRP conjugate:\u003c\/strong\u003e Direct chromogenic (TMB\/OPD) or chemiluminescent (ECL) detection. Quench endogenous peroxidase in tissue applications. No secondary required.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eBiological background\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003eFCER2\u003c\/strong\u003e (also known as Blast 2, BLAST-2, Blast2, C type lectin domain family 4 member J, C-type lectin domain family 4, C-type lectin domain family 4 member J, C-type lectin domain family 4, member J, CD 23, CD 23A, CD23, CD23 antigen, CD23A, CLEC 4J, CLEC4J, Fc epsilon receptor II, Fc epsilon RII, Fc fragment of IgE, Fc fragment of IgE low affinity II receptor for, Fc fragment of IgE receptor II, Fc fragment of IgE, low affinity II, receptor for (CD23), Fc of IgE, Fc of IgE, low affinity II, receptor for (CD23), Fc receptor IgE low affinity II alpha polypeptide, Fc receptor, IgE, low affinity II, alpha polypeptide, isoform CRA_a, Fc-epsilon-RII, FCE 2, FCE2, FCER 2, Fcer2, FCER2_HUMAN, FCER2A, FceRII, IgE binding factor, IgE receptor lymphocyte, IgE-binding factor, IGEBF, Immunoglobulin E binding factor, Immunoglobulin E receptor, Immunoglobulin E receptor, low affinity II, Immunoglobulin E-binding factor, Immunoglobulin epsilon chain, LEUKOCYTE ANTIGEN CD23, Low Affinity IgE Receptor, Low affinity immunoglobulin epsilon Fc receptor, Low affinity immunoglobulin epsilon Fc receptor membrane bound form, Low affinity immunoglobulin epsilon Fc receptor soluble form, Ly-42, Ly42, Lymphocyte antigen CD23, Lymphocyte IgE receptor, MGC93219) is a protein studied in Human systems. Expression levels, subcellular localization, and post-translational modifications vary across cell types, tissues, and disease states — factors that influence antibody-based detection outcomes. Researchers are encouraged to consult UniProt, NCBI Gene, and primary literature for current annotation of FCER2 biology relevant to immunology.\u003c\/p\u003e\n\n\u003ch2\u003eCommon research applications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e Sandwich or indirect ELISA quantifies soluble\/secreted target in biological fluids or cell culture supernatants.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eIsotype controls:\u003c\/strong\u003e Include IgG from Rabbit at equivalent concentration to assess non-specific background.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eCross-reactivity\/isoforms:\u003c\/strong\u003e Polyclonal preparations may cross-react with related proteins or isoforms — validate with siRNA KD, KO lysate, or recombinant protein controls.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eMatrix effects:\u003c\/strong\u003e Sample matrix (serum, plasma, lysate, homogenate) may influence performance; pilot dilution linearity and spike-recovery experiments before full quantitative studies.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eSpecies reactivity:\u003c\/strong\u003e Confirmed for Human; extrapolation to untested species requires empirical validation.\u003c\/li\u003e\n\u003c\/ul\u003e\n\n\u003cp\u003e\u003cem\u003eThis product is a polyclonal antibody purified from rabbit antiserum.\u003c\/em\u003e\u003c\/p\u003e\n\n\u003c!-- Sources (internal):\n- UniProt: https:\/\/www.uniprot.org\n- NCBI Gene: https:\/\/www.ncbi.nlm.nih.gov\/gene\n- Cusabio: https:\/\/www.cusabio.com\n- Human Protein Atlas: https:\/\/www.proteinatlas.org\n- PubMed: https:\/\/pubmed.ncbi.nlm.nih.gov\n--\u003e","brand":"CUSABIO TECHNOLOGY LLC","offers":[{"title":"50 ug","offer_id":53238725050733,"sku":"CSB-PA02219B0Rb-50UG","price":170.98,"currency_code":"USD","in_stock":true},{"title":"100 ug","offer_id":53238846980461,"sku":"CSB-PA02219B0Rb-100UG","price":307.97,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/CSB-PA02219A0Rb-IHC-1_d3c73c04-1f78-4d03-b3bb-3fe2a5aa34ce.jpg?v=1782237649","url":"https:\/\/www.ebiohippo.com\/products\/fcer2-antibody-hrp-conjugated-bha10505422","provider":"BioHippo","version":"1.0","type":"link"}