{"product_id":"ffpe-dna-extraction-kit-bht15200015","title":"FFPE DNA Extraction Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eDNA Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis product is suitable for separating and purifying total DNA (including genomic DNA, mitochondrial DNA and any viral DNA present) from 3–8 tissue sections of 10 µm, with a total area of less than 250 mm². The DNA is adsorbed to the purification column, and the degraded protein and PCR inhibitors are removed by the specific washing solution, and the final DNA product is eluted with Buffer TE, so it can be used in various molecular biology experiments such as PCR, sequencing, and enzyme digestion.\u003c\/p\u003e\n\u003cp\u003eSections are deparaffinised with xylene using a 10 s vortex, washed with ethanol and air-dried, then digested with Proteinase K at 56°C for 1 h and heated at 90°C for 1 h. After addition of the binding buffers the lysate is bound to Adsorption Column G at 12,000 rpm for 30 s, washed with Buffer RP and Buffer WB, dried at 14,000 rpm for 1 min and eluted with 60–100 µL of Buffer TE at 56°C. The expected purity is OD260\/OD280 1.7–1.9.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSpin column (Adsorption Column G)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample type\u003c\/th\u003e\n\u003ctd\u003eFormalin-fixed paraffin-embedded animal tissue sections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003e3–8 sections of 10 µm, area less than 250 mm²\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eNucleic acids recovered\u003c\/th\u003e\n\u003ctd\u003eGenomic DNA, mitochondrial DNA and possible viral DNA\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003ePurity (OD260\/OD280)\u003c\/th\u003e\n\u003ctd\u003e1.7–1.9\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e60–100 µL Buffer TE at 56°C\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteinase K\u003c\/td\u003e\n\u003ctd\u003e1.2 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GA1\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GA2\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RP\u003c\/td\u003e\n\u003ctd\u003e28 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer WB\u003c\/td\u003e\n\u003ctd\u003e32 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer TE\u003c\/td\u003e\n\u003ctd\u003e12 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eAdsorption Column G\u003c\/td\u003e\n\u003ctd\u003e50 set\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTotal DNA extraction from FFPE tissue sections\u003c\/li\u003e\n\u003cli\u003eRecovery of genomic, mitochondrial and possible viral DNA\u003c\/li\u003e\n\u003cli\u003ePCR from archived tissue\u003c\/li\u003e\n\u003cli\u003eSequencing and enzyme digestion\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eProteinase K is stored at -20°C for 2 years. The other components are stored at room temperature for 1 year. Storage at 2–8°C extends validity beyond 2 years.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eDiscard the first 2–3 tissue slices before collecting sections for extraction.\u003c\/li\u003e\n\u003cli\u003eAvoid contact between the solutions and the column nozzle.\u003c\/li\u003e\n\u003cli\u003eDo not omit the drying step, as residues compromise PCR.\u003c\/li\u003e\n\u003cli\u003eFor low yield, extend the digestion time and increase the sample volume.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP016\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200015\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"50 T","offer_id":53965513425261,"sku":"EP016-50T","price":364.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 5","offer_id":53965515391341,"sku":"EP016-50TX5","price":0.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/EP016-a-1.jpg?v=1789419186","url":"https:\/\/www.ebiohippo.com\/products\/ffpe-dna-extraction-kit-bht15200015","provider":"BioHippo","version":"1.0","type":"link"}