{"product_id":"ffpe-rna-extraction-kit-bht15200025","title":"FFPE RNA Extraction Kit","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc .bhp-spec-table{width:100%;border-collapse:collapse;margin:12px 0 18px}.bhp-desc .bhp-spec-table th{width:36%;text-align:left;padding:8px 12px;background:#e8f0fb;color:#003366;font-weight:600;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-spec-table td{padding:8px 12px;vertical-align:top;border:1px solid #ccd9f0}.bhp-desc .bhp-qc-badge{display:inline-block;background:#e6f4ea;color:#1a7a3c;border:1px solid #a8d5b5;border-radius:4px;padding:6px 14px;font-size:14px;font-weight:600}.bhp-desc .bhp-warning-box{background:#fff8e6;border-left:4px solid #e6a817;padding:10px 14px;margin:10px 0 14px;border-radius:0 4px 4px 0}.bhp-desc .bhp-license-box{background:#f5f5f5;border:1px solid #ddd;border-radius:4px;padding:12px 16px;font-size:14px;color:#444}.bhp-desc .bhp-regulatory-row{display:flex;gap:16px;flex-wrap:wrap;margin:6px 0 10px}.bhp-desc .bhp-reg-item{background:#f0f6ff;border:1px solid #ccd9f0;border-radius:4px;padding:6px 12px;font-size:14px}.bhp-desc .bhp-reg-item strong{color:#003366;display:block;font-size:12px;text-transform:uppercase;letter-spacing:.5px}.bhp-desc .bhp-cat-pill{display:inline-block;background:#003366;color:#fff;border-radius:20px;padding:3px 12px;font-size:13px;margin-bottom:10px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}.bhp-desc .bhp-comp-table th{width:auto}\u003c\/style\u003e\n\u003cspan class=\"bhp-cat-pill\"\u003eRNA Extraction Kits\u003c\/span\u003e\u003ch2\u003eProduct Overview\u003c\/h2\u003e\n\u003cp\u003eThis kit is suitable for effectively purifying total RNA from formalin-fixed and paraffin-embedded tissues. It is suitable for extracting high-purity total RNA from wax-embedded tissues or sections weighing less than 30 mg. This kit does not require the use of phenol\/chloroform extraction and isopropanol precipitation. Multiple samples can be extracted within one hour. This product uses specially optimized lysis buffer and proteinase K to release RNA from formalin-fixed or tissue section samples. No overnight operation is required; after digestion of the sample, incubation at a higher temperature removes the inhibitory effect caused by formalin cross-linking, effectively releasing RNA from tissue sections while avoiding damage to the integrity of RNA; the optimized buffer system enables RNA in the lysis buffer to specifically bind to the silica gel adsorption membrane, while other contaminants can flow through the membrane; it can be effectively removed through the rinsing step, and the eluted RNA can be directly used for RT-PCR, Real-Time PCR and blotting analysis and other experiments.\u003c\/p\u003e\n\u003cp\u003eParaffin is removed by one of two procedures (Plan A or Plan B in the manual). Buffer GTL and Proteinase K are then added and the sample is incubated at 56°C for 15 minutes followed by 80°C for 15 minutes. After cooling on ice and centrifugation, Buffer GL and ethanol are added and the lysate is bound to the Spin Column DM. An optional DNase I treatment is available at this step, followed by two Buffer RW2 washes, drying and elution in 20–50 µL of RNase-Free Water.\u003c\/p\u003e\n\u003ch2\u003eSpecifications\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eFormat\u003c\/th\u003e\n\u003ctd\u003eSpin column (Spin Columns DM, silica gel adsorption membrane)\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample type\u003c\/th\u003e\n\u003ctd\u003eFormalin-fixed paraffin-embedded tissue or sections\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSample input\u003c\/th\u003e\n\u003ctd\u003eLess than 30 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eProcessing time\u003c\/th\u003e\n\u003ctd\u003eMultiple samples within one hour\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eChemistry\u003c\/th\u003e\n\u003ctd\u003eNo phenol\/chloroform extraction, isopropanol precipitation or overnight step\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eSpecial treatment\u003c\/th\u003e\n\u003ctd\u003eOptional DNase I; 1000 U with 10× Reaction Buffer supplied\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003cth\u003eElution volume\u003c\/th\u003e\n\u003ctd\u003e20–50 µL RNase-Free Water\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eKit Components\u003c\/h2\u003e\n\u003ctable class=\"bhp-spec-table bhp-comp-table\"\u003e\n\u003ctr\u003e\n\u003cth\u003eComponent\u003c\/th\u003e\n\u003cth\u003e50 T\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eDNase I\u003c\/td\u003e\n\u003ctd\u003e1000 U\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003e10× Reaction Buffer\u003c\/td\u003e\n\u003ctd\u003e1000 µL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer DS\u003c\/td\u003e\n\u003ctd\u003e30 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GTL\u003c\/td\u003e\n\u003ctd\u003e15 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer GL\u003c\/td\u003e\n\u003ctd\u003e25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteinase K\u003c\/td\u003e\n\u003ctd\u003e12.5 mg\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eProteinase K Storage Buffer\u003c\/td\u003e\n\u003ctd\u003e1.25 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RW1\u003c\/td\u003e\n\u003ctd\u003e40 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBuffer RW2 (concentrate)\u003c\/td\u003e\n\u003ctd\u003e11 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-Free Water\u003c\/td\u003e\n\u003ctd\u003e10 mL\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSpin Columns DM with Collection Tubes\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eRNase-Free Centrifuge Tubes (1.5 mL)\u003c\/td\u003e\n\u003ctd\u003e50\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/table\u003e\n\u003ch2\u003eApplications\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eTotal RNA purification from formalin-fixed paraffin-embedded tissue\u003c\/li\u003e\n\u003cli\u003eRNA extraction from wax-embedded sections under 30 mg\u003c\/li\u003e\n\u003cli\u003eRT-PCR and Real-Time PCR from archived tissue\u003c\/li\u003e\n\u003cli\u003eBlotting analysis\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eStorage \u0026amp; Handling\u003c\/h2\u003e\n\u003cp\u003eDNase I and the 10× Reaction Buffer are stored at -20°C; the other components are stored at room temperature (15–30°C). The shelf life is 1 year at room temperature.\u003c\/p\u003e\n\u003ch2\u003eUsage Notes\u003c\/h2\u003e\n\u003cul\u003e\n\u003cli\u003eUse RNase-free materials and disposable masks and gloves.\u003c\/li\u003e\n\u003cli\u003eSamples should be fixed in 4%–10% formalin for 14–24 hours.\u003c\/li\u003e\n\u003cli\u003eAvoid long-term room temperature storage of Proteinase K.\u003c\/li\u003e\n\u003cli\u003eEthanol is required at the binding step and to prepare Buffer RW2; it is not supplied.\u003c\/li\u003e\n\u003cli\u003eCheck the buffers for crystals before use.\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2\u003eSafety \u0026amp; Regulatory\u003c\/h2\u003e\n\u003cdiv class=\"bhp-warning-box\"\u003e\u003cp\u003eFor research use only. Not intended for clinical, diagnostic or therapeutic use. Follow the manufacturer's manual and standard laboratory safety practice.\u003c\/p\u003e\u003c\/div\u003e\n\u003cdiv class=\"bhp-regulatory-row\"\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eGrade\u003c\/strong\u003eResearch Use Only\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eManufacturer\u003c\/strong\u003eELK Biotechnology\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eCatalog No.\u003c\/strong\u003eEP031\u003c\/div\u003e\n\u003cdiv class=\"bhp-reg-item\"\u003e\n\u003cstrong\u003eBioHippo SKU\u003c\/strong\u003eBHT15200025\u003c\/div\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e","brand":"ELK Biotechnology","offers":[{"title":"50 T","offer_id":53965512442221,"sku":"EP031-50T","price":390.0,"currency_code":"USD","in_stock":true},{"title":"50 T x 10","offer_id":53965515227501,"sku":"EP031-50TX10","price":0.0,"currency_code":"USD","in_stock":true}],"url":"https:\/\/www.ebiohippo.com\/products\/ffpe-rna-extraction-kit-bht15200025","provider":"BioHippo","version":"1.0","type":"link"}