{"product_id":"fgd5-antibody-fyve-rhogef-and-ph-domain-containing-protein-5-bha17135288","title":"FGD5 Antibody \/ FYVE, RhoGEF and PH domain-containing protein 5","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eFGD5 Antibody \/ FYVE, RhoGEF and PH domain-containing protein 5 is a anti-FGD5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e FGD5\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eThe FGD5 antibody targets FYVE, RhoGEF, and PH domain-containing protein 5, a guanine nucleotide exchange factor encoded by the FGD5 gene. This protein belongs to the FGD family of Rho guanine nucleotide exchange factors (GEFs) that activate CDC42, a small GTPase involved in cytoskeletal organization, vesicle trafficking, and angiogenesis. FYVE, RhoGEF, and PH domain-containing protein 5 is enriched in endothelial cells and plays a critical role in vascular development and remodeling. The FGD5 antibody enables detection and quantification of this signaling molecule, supporting research into endothelial cell biology and vascular homeostasis.\u003cbr\u003e\u003cbr\u003eFGD5 contains multiple conserved domains, including a FYVE domain that binds phosphatidylinositol 3-phosphate, a Dbl homology (DH) domain that catalyzes GTP loading of CDC42, and a pleckstrin homology (PH) domain that anchors the protein to membranes. These domains coordinate spatial activation of CDC42 at specific cellular sites, promoting actin cytoskeletal rearrangements necessary for cell migration and tube formation. The FGD5 antibody provides a key reagent for examining how this protein localizes to endosomes and plasma membrane regions during angiogenic signaling.\u003cbr\u003e\u003cbr\u003eEndothelial FGD5 regulates cell polarity, barrier integrity, and lumen formation by activating CDC42 and downstream effectors such as PAK and N-WASP. Its expression is induced by vascular growth factors, including VEGF and FGF, which drive endothelial morphogenesis. The FGD5 antibody supports studies into these processes, helping delineate how Rho GTPase signaling orchestrates angiogenic sprouting and vessel stabilization. Loss of FGD5 function leads to impaired vascular network formation, highlighting its essential developmental role.\u003cbr\u003e\u003cbr\u003eBeyond angiogenesis, FYVE, RhoGEF, and PH domain-containing protein 5 contributes to intracellular trafficking and signal transduction in other tissues, including kidney and brain. Abnormal FGD5 expression has been linked to vascular pathologies, diabetic retinopathy, and cancer progression, where altered endothelial behavior supports tumor angiogenesis. The FGD5 antibody allows researchers to quantify expression changes under pathological conditions and explore its regulatory influence on endothelial plasticity.\u003cbr\u003e\u003cbr\u003eThe FGD5 antibody performs well in western blotting, immunofluorescence, and immunohistochemistry, yielding distinct cytoplasmic and membrane-associated staining.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=FGD5 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FGD5 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=FGD5 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/FGD5 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=FGD5+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047287447917,"sku":"FY12385","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_cdc16aa2-1c56-44c8-9a34-1f4ace3711c8.jpg?v=1782236993","url":"https:\/\/www.ebiohippo.com\/products\/fgd5-antibody-fyve-rhogef-and-ph-domain-containing-protein-5-bha17135288","provider":"BioHippo","version":"1.0","type":"link"}