{"product_id":"fluorescence-and-luciferase-dual-reporter-lentivirus-bhv19400181","title":"Fluorescence and Luciferase Dual Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eTracking the location, abundance, and behavior of defined cell populations is fundamental to cancer and immunology research. Bioluminescence imaging with luciferase enables sensitive, longitudinal monitoring of cell number in living animals, while fluorescent proteins allow single-cell identification by microscopy and flow cytometry. Combining both modalities in the same cell provides complementary readouts: luciferase for whole-body imaging of tumor growth and metastasis, and fluorescence for ex vivo histology, FACS-based isolation, and analysis of tumor-infiltrating immune cells. Stable, division-resistant labeling is essential for accurate longitudinal preclinical studies of tumor biology and the tumor microenvironment.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe Fluorescence and Luciferase Dual Reporter Lentivirus constitutively co-expresses a luminescent reporter (firefly or secreted Gaussia luciferase) and a fluorescent protein (GFP or RFP) from a single transcript, using self-cleaving peptides for independent, equimolar protein production. Expression is driven by a CMV, PGK, or EF1α promoter, and a selection marker (puromycin or blasticidin) supports stable cell line generation. Stable integration ensures persistent dual-reporter expression, enabling longitudinal bioluminescence imaging of tumor growth and metastasis in vivo and parallel fluorescence-based identification, gating, or FACS sorting of tumor cells for flow cytometry, scRNA-seq, and proteomics. Multiple vector layouts optimize for stable line establishment or primary-cell labeling. Supplied as ultra-purified, high-titer lentiviral particles suitable for in vitro and in vivo use, including transduction of primary and cryopreserved cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis lentivirus constitutively co-expresses a luciferase reporter and a fluorescent protein (GFP, RFP, Luc) from a strong CMV or EF1α promoter, using a self-cleaving P2A peptide to ensure equimolar, independent production of both reporters. A selection marker (Blasticidin, Puromycin) is co-encoded for stable cell line generation.\u003c\/p\u003e\n\u003cp\u003eThe stable lentiviral integration ensures persistent, division-stable reporter expression in the target cell line, enabling longitudinal bioluminescence imaging (IVIS) of tumor burden in mouse models and parallel fluorescence-based readouts for ex vivo analysis. The dual-modality design provides built-in redundancy — luciferase for high-sensitivity whole-body imaging and fluorescence for histology, flow cytometry, and FACS — within a single stably engineered cell line.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"CMV-Luc-GFP-Puro \/ Puromycin \/ 3.5x10^6","offer_id":53251625812333,"sku":"LFV-0001-01","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Luc-RFP-Puro \/ Puromycin \/ 3.5x10^6","offer_id":53362028544365,"sku":"LFV-0001-02","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Luc-GFP-BSD \/ Blasticidin \/ 3.5x10^6","offer_id":53362028577133,"sku":"LFV-0001-03","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-Luc-RFP-BSD \/ Blasticidin \/ 3.5x10^6","offer_id":53362028609901,"sku":"LFV-0001-04","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GFP-Puro-Luc \/ Puromycin \/ 3.5x10^6","offer_id":53362028642669,"sku":"LFV-0001-05","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-RFP-Puro-Luc \/ Puromycin \/ 3.5x10^6","offer_id":53362028675437,"sku":"LFV-0001-06","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GFP-BSD-Luc \/ Blasticidin \/ 3.5x10^6","offer_id":53362028708205,"sku":"LFV-0001-07","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-RFP-BSD-Luc \/ Blasticidin \/ 3.5x10^6","offer_id":53362028740973,"sku":"LFV-0001-08","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1α-Luc-GFP-Puro \/ Puromycin \/ 3.5x10^6","offer_id":53362028773741,"sku":"LFV-0001-09","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1α-Luc-RFP-BSD \/ Blasticidin \/ 3.5x10^6","offer_id":53362028806509,"sku":"LFV-0001-10","price":595.0,"currency_code":"USD","in_stock":true},{"title":"PGK-GFP-P2A-Luc \/ N\/A \/ 5x10^6","offer_id":53362028839277,"sku":"LFV-0001-11","price":595.0,"currency_code":"USD","in_stock":true},{"title":"PGK-RFP-P2A-Luc \/ N\/A \/ 5x10^6","offer_id":53362028872045,"sku":"LFV-0001-12","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1α-Luc-P2A-GFP \/ N\/A \/ 5x10^6","offer_id":53362028904813,"sku":"LFV-0001-13","price":595.0,"currency_code":"USD","in_stock":true},{"title":"EF1α-Luc-P2A-RFP \/ N\/A \/ 5x10^6","offer_id":53362028937581,"sku":"LFV-0001-14","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-RFP-BSD \/ Blasticidin \/ 5x10^6","offer_id":53362028970349,"sku":"LFV-0001-16","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-GFP-Puro \/ Puromycin \/ 5x10^6","offer_id":53362029003117,"sku":"LFV-0001-15","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-RFP-Puro \/ Puromycin \/ 5x10^6","offer_id":53362029035885,"sku":"LFV-0001-18","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-GFP-BSD \/ Blasticidin \/ 5x10^6","offer_id":53362029068653,"sku":"LFV-0001-17","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-rFLuc-RFP-BSD \/ Blasticidin \/ 2x10^6","offer_id":53362029101421,"sku":"LFV-0001-19","price":595.0,"currency_code":"USD","in_stock":true},{"title":"CMV-GLuc-ZsG-BSD \/ Blasticidin \/ 5x10^6","offer_id":53362029134189,"sku":"LFV-0001-20","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/LucFluo-Fig1-JPG-3.jpg?v=1776934330","url":"https:\/\/www.ebiohippo.com\/products\/fluorescence-and-luciferase-dual-reporter-lentivirus-bhv19400181","provider":"BioHippo","version":"1.0","type":"link"}