{"product_id":"foxa1-2-reporter-lentivirus-bhv19400229","title":"FOXA1\/2 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eFOXA1 and FOXA2, also known as HNF-3A and HNF-3B, are forkhead-box pioneer transcription factors that bind compacted chromatin and open it to enable the recruitment of additional transcription factors. They are central regulators of endoderm-derived organ development, directing the formation and function of liver, pancreas, and lung. In adult tissues FOXA1\/2 control glucose and lipid metabolism and act as cofactors for nuclear hormone receptors, shaping their genomic binding. The factors also influence cell proliferation, differentiation, migration, invasion, and drug resistance. Altered FOXA1\/2 activity is implicated in metabolic disease and in several cancers, where their pioneering function affects lineage identity and tumor behavior, making them important targets in developmental biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FOXA1\/2 Reporter Lentivirus is a transcription factor reporter system that detects transcriptional activity mediated by FOXA1 and FOXA2 in mammalian cells. The construct incorporates tandem repeats of consensus DNA-binding elements from the human protein C promoter, placed upstream of a minimal promoter driving a fluorescent or luminescent reporter, with an optimized upstream enhancer that maximizes signal-to-noise and provides specific readout of FOXA1\/2 activity. A constitutive drug selection marker (Blasticidin, Hygromycin, Puromycin, or Zeocin) enables generation of stable polyclonal reporter cell lines suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, the product is well suited to studying FOXA1\/2 transcriptional activity in primary and difficult-to-transfect cells for developmental and cancer research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXA1\/2 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"FOXA1\/2-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251617554797,"sku":"LTV-0122-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362086445421,"sku":"LTV-0122-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362086478189,"sku":"LTV-0122-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FOXA1\/2-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362086510957,"sku":"LTV-0122-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362086543725,"sku":"LTV-0122-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362086576493,"sku":"LTV-0122-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362086609261,"sku":"LTV-0122-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FOXA1\/2-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362086642029,"sku":"LTV-0122-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FOXA1\/2-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362086674797,"sku":"LTV-0122-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FOXA1\/2-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362086707565,"sku":"LTV-0122-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FOXA1\/2-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362086740333,"sku":"LTV-0122-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FOXA1-2-Reporter-Figure.jpg?v=1776934213","url":"https:\/\/www.ebiohippo.com\/products\/foxa1-2-reporter-lentivirus-bhv19400229","provider":"BioHippo","version":"1.0","type":"link"}