{"product_id":"foxc1-reporter-lentivirus-bhv19400249","title":"FOXC1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eFOXC1 is a forkhead-box transcription factor that binds specific DNA elements to regulate gene expression during development. It is expressed predominantly in the heart, kidney, cartilage, and sternum, where it controls genes such as Col10a1, PTHrP, Gli1, and Ptch1 in chondrocytes. These targets are synergistically stimulated by co-expressed Gli2 and through Ihh-Gli2 signaling during endochondral ossification, linking FOXC1 to skeletal development. Pathological FOXC1 missense mutations, such as those found in Axenfeld-Rieger syndrome, impair chondrocyte proliferation and maturation and disrupt the Gli2-FOXC1 signaling association. FOXC1 is therefore an important factor in developmental biology and skeletal disease research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FOXC1 Reporter Lentivirus is a lentiviral reporter system that detects transcriptional activity mediated by FOXC1 in mammalian cells. The construct contains tandem repeats of FOXC1 DNA-binding elements derived from the mouse Col10a1 promoter, driving expression of a fluorescent (GFP, RFP, BFP, mCherry, EGFP, d2GFP) or luminescent (firefly, Renilla, or Gaussia luciferase) reporter. It provides a sensitive readout of FOXC1-driven transcription and the signaling, including Gli2 and Ihh-Gli2 inputs, that modulates it. A range of antibiotic selection markers (blasticidin, hygromycin, puromycin, zeocin) supports establishment of stable reporter cell lines. Supplied as lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, it ensures efficient transduction of difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXC1 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"FOXC1-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251618668909,"sku":"LTV-0142-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362092212589,"sku":"LTV-0142-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362092245357,"sku":"LTV-0142-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FOXC1-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362092278125,"sku":"LTV-0142-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362092310893,"sku":"LTV-0142-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362092343661,"sku":"LTV-0142-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362092376429,"sku":"LTV-0142-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FOXC1-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362092409197,"sku":"LTV-0142-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FOXC1-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362092441965,"sku":"LTV-0142-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FOXC1-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362092474733,"sku":"LTV-0142-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FOXC1-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362092507501,"sku":"LTV-0142-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FOXC1-TAG-PURO-6P.jpg?v=1776934149","url":"https:\/\/www.ebiohippo.com\/products\/foxc1-reporter-lentivirus-bhv19400249","provider":"BioHippo","version":"1.0","type":"link"}