{"product_id":"foxc2-antibody-forkhead-box-protein-c2-bha17135359","title":"FOXC2 Antibody \/ Forkhead box protein C2","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eFOXC2 Antibody \/ Forkhead box protein C2 is a anti-FOXC2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e FOXC2\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC\/IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eFOXC2 antibody detects Forkhead box protein C2, a transcription factor belonging to the forkhead family characterized by a conserved DNA-binding forkhead domain. FOXC2 plays essential roles in embryonic development, angiogenesis, lymphangiogenesis, adipose differentiation, and epithelial-mesenchymal transition (EMT). It regulates transcriptional programs that control cell fate and morphogenesis in multiple tissues. The FOXC2 antibody is widely employed to investigate vascular development, cancer metastasis, and metabolic regulation, as FOXC2 activity is tightly linked to both physiological and pathological tissue remodeling.\u003cbr\u003e\u003cbr\u003eFOXC2 is encoded by the FOXC2 gene located on human chromosome 16q24.1. It binds to promoter regions of target genes containing the forkhead consensus sequence and activates transcription of genes involved in cell migration and morphogenesis. In the vascular system, FOXC2 controls differentiation of lymphatic endothelial cells and formation of lymphatic valves. Mutations in FOXC2 cause lymphedema-distichiasis syndrome, characterized by lymphatic dysfunction and abnormal eyelash growth.\u003cbr\u003e\u003cbr\u003eThe FOXC2 antibody is frequently used in developmental and cancer research to detect protein expression in tissue sections and cultured cells. Western blot analysis typically reveals a 50�55 kDa nuclear protein, while immunohistochemistry demonstrates nuclear staining in endothelial and mesenchymal cells. In cancer biology, FOXC2 serves as a key regulator of EMT and is upregulated in aggressive tumors, where it promotes invasion, metastasis, and resistance to chemotherapy.\u003cbr\u003e\u003cbr\u003eFOXC2 also contributes to adipose tissue differentiation and energy homeostasis by regulating genes that determine brown adipocyte lineage. It functions in cooperation with transcription factors such as PPARgamma and PRDM16. Beyond developmental and metabolic functions, FOXC2 is implicated in cardiac valve formation and osteogenesis.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=FOXC2 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FOXC2 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=FOXC2 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/FOXC2 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=FOXC2+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047286432109,"sku":"FY12456","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_d4d130cf-b9eb-4fe2-aeaa-1ead10af4b5d.jpg?v=1782236993","url":"https:\/\/www.ebiohippo.com\/products\/foxc2-antibody-forkhead-box-protein-c2-bha17135359","provider":"BioHippo","version":"1.0","type":"link"}