{"product_id":"foxc2-reporter-lentivirus-bhv19400057","title":"FOXC2 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eFOXC2 belongs to the Forkhead box family of transcription factors, which share a conserved DNA-binding domain but differ in flanking sequences that determine target specificity. FOXC2 controls the expression of genes involved in normal development, including the formation of veins, lymphatic vessels, lungs, eyes, kidneys, the urinary tract, and the cardiovascular system. It is a key regulator of lymphatic vascular development and immune cell transport. FOXC2 is also linked to epithelial-mesenchymal transition and is studied in the context of cancer progression and metastasis, making it relevant to developmental biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FOXC2 Reporter Lentivirus is a transcription factor reporter system built with tandem repeats of FOXC2 DNA-binding elements designed to preferentially read out FOXC2 activity over other Forkhead transcription factors. The elements drive a reporter gene downstream of a minimal promoter, giving a sensitive fluorescent or luminescent readout of FOXC2 transcriptional activity and the signaling pathways that activate it. Reporter options include EGFP, GFP, mCherry, RFP, d2GFP, and firefly luciferase, with optional blasticidin or puromycin selection for stable polyclonal cell line generation. Particles are purified by PEG precipitation and sucrose gradient centrifugation and efficiently transduce difficult-to-transfect cells, including primary and thawed cells.\u003c\/p\u003e\n\u003cp\u003eIt supports stable reporter cell line generation for studying FOXC2 activity in development and disease.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a d2GFP, EGFP, Firefly Luc, mCherry, GFP, RFP, Luc reporter gene under the control of tandem consensus response elements specific for the FOXC2 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Puromycin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251617063277,"sku":"LTV-0061-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53310893818221,"sku":"LTV-0061-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53310893850989,"sku":"LTV-0061-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53310893883757,"sku":"LTV-0061-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53310893916525,"sku":"LTV-0061-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53310893949293,"sku":"LTV-0061-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/TF-Reporter-Vector-circular_d9a7d442-c0b8-4118-b72a-b537f2ec0887.jpg?v=1776934139","url":"https:\/\/www.ebiohippo.com\/products\/foxc2-reporter-lentivirus-bhv19400057","provider":"BioHippo","version":"1.0","type":"link"}