{"product_id":"foxd1-reporter-lentivirus-bhv19400226","title":"FOXD1 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eFOXD1 is a forkhead box transcription factor with a conserved winged-helix DNA-binding domain that regulates genes essential for developmental patterning and tissue maintenance. It has well-defined roles in kidney and retina development, where it directs stromal cell behavior, as well as in lymphatic vessel formation and embryo implantation. Among its target genes is placental growth factor, a VEGF family member involved in angiogenesis. FOXD1 has been associated with several human conditions, including cancer and recurrent pregnancy loss, where altered FOXD1 activity affects tissue development and homeostasis. These functions make FOXD1-dependent transcription an informative readout in developmental and disease biology research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FOXD1 Reporter Lentivirus is a transcription-factor reporter system designed to preferentially detect FOXD1 transcriptional activity in human and mouse cells. The construct uses tandem repeats of the FOXD1 DNA-binding element derived from the human placental growth factor promoter, a validated downstream target, to drive a fluorescent or luminescent reporter, with options including GFP, RFP, mCherry, BFP2, and luciferase configurations. A constitutively expressed selection marker supports stable polyclonal cell line generation.\u003c\/p\u003e\n\u003cp\u003eReadout is by microscopy, flow cytometry, or luminometry. Applications include studying FOXD1 in kidney and retina development, lymphatic biology, and related disease processes. Particles are purified by PEG precipitation and sucrose gradient centrifugation for transduction of primary and thawed cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FOXD1 transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GFP \/ Puromycin \/ 5x10^6","offer_id":53251616670061,"sku":"LTV-0117-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Blasticidin \/ 5x10^6","offer_id":53362027790701,"sku":"LTV-0117-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Puromycin \/ 2x10^6","offer_id":53362027823469,"sku":"LTV-0117-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Firefly Luc \/ Blasticidin \/ 2x10^6","offer_id":53362027856237,"sku":"LTV-0117-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GFP \/ Blasticidin \/ 5x10^6","offer_id":53362027889005,"sku":"LTV-0117-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"RFP \/ Puromycin \/ 5x10^6","offer_id":53362027921773,"sku":"LTV-0117-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/FOXD1-TAG-Puro-Fig1.jpg?v=1776934220","url":"https:\/\/www.ebiohippo.com\/products\/foxd1-reporter-lentivirus-bhv19400226","provider":"BioHippo","version":"1.0","type":"link"}