{"product_id":"foxi1-antibody-forkhead-box-protein-i1-bha17136201","title":"FOXI1 Antibody \/ Forkhead box protein I1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eFOXI1 Antibody \/ Forkhead box protein I1 is a anti-FOXI1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Nucleolus, Vesicles.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e FOXI1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF, FACS, ELISA\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eFOXI1 antibody targets Forkhead box protein I1, a member of the FOX family of transcription factors characterized by a conserved forkhead or 'winged helix' DNA-binding domain. The FOXI1 gene, located on chromosome 5q35.1, encodes a nuclear transcription factor that regulates ion transport and epithelial differentiation, particularly in the inner ear, kidney, and epididymis. FOXI1 controls expression of genes involved in acid-base balance and fluid homeostasis, including the vacuolar H+-ATPase subunit and pendrin (SLC26A4), a chloride\/bicarbonate exchanger essential for maintaining luminal pH.\u003cbr\u003e\u003cbr\u003eStructurally, FOXI1 contains a 110-amino acid forkhead domain that interacts with specific DNA motifs to modulate transcriptional activity. It functions as a transcriptional activator for genes expressed in intercalated cells of the distal nephron and cochlear epithelial cells. In the inner ear, FOXI1 contributes to endolymphatic ion transport and hearing development. In the kidney, loss of FOXI1 disrupts acid secretion, resulting in distal renal tubular acidosis (dRTA). Genetic mutations in FOXI1 have been linked to autosomal recessive dRTA and sensorineural hearing loss, demonstrating its dual role in renal and auditory physiology.\u003cbr\u003e\u003cbr\u003eFOXI1 also participates in epithelial polarity regulation and may influence Wnt and Notch signaling pathways during organogenesis. Dysregulation of FOXI1 expression has been reported in certain cancers, where it may contribute to altered epithelial transport and cell differentiation. Functional studies suggest FOXI1 acts as a transcriptional regulator for ion transport proteins and can modulate developmental genes associated with morphogenesis of the ear and kidney.\u003cbr\u003e\u003cbr\u003eImmunohistochemical staining using FOXI1 antibody reveals nuclear localization in renal intercalated cells, cochlear epithelium, and reproductive tissues. FOXI1 antibody from\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eELISA:\u003c\/strong\u003e support antibody-based quantification in assay formats where applicable.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=FOXI1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FOXI1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=FOXI1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/FOXI1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=FOXI1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047321756013,"sku":"FY13299","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_f67cca42-d53d-4ddf-93e5-56041e4ea2ed.jpg?v=1782237088","url":"https:\/\/www.ebiohippo.com\/products\/foxi1-antibody-forkhead-box-protein-i1-bha17136201","provider":"BioHippo","version":"1.0","type":"link"}