{"product_id":"fpgs-antibody-folylpolyglutamate-synthase-bha17135905","title":"FPGS Antibody \/ Folylpolyglutamate synthase","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eFPGS Antibody \/ Folylpolyglutamate synthase is a anti-FPGS Rabbit antibody Recombinant Rabbit Monoclonal clone 29F90 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e FPGS\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 29F90, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eFPGS antibody detects Folylpolyglutamate synthase, encoded by the FPGS gene. Folylpolyglutamate synthase is a key enzyme in folate metabolism that catalyzes the addition of glutamate residues to folate and antifolate compounds, converting them into polyglutamated forms that are retained within cells. These polyglutamates are essential for nucleotide biosynthesis and methylation reactions, as they serve as cofactors in one-carbon metabolism. FPGS antibody provides researchers with a powerful tool to study folate biology, cancer metabolism, and drug resistance.\u003cbr\u003e\u003cbr\u003eFolylpolyglutamate synthase is localized to both mitochondria and cytosol, ensuring polyglutamation occurs in multiple compartments. This enzyme plays an indispensable role in maintaining pools of tetrahydrofolate derivatives required for DNA synthesis and repair. Research using FPGS antibody has revealed that reduced enzyme activity decreases intracellular folate retention and disrupts nucleotide biosynthesis, leading to impaired proliferation. These findings explain why mutations or dysregulation of FPGS contribute to megaloblastic anemia and developmental defects.\u003cbr\u003e\u003cbr\u003eIn oncology, FPGS is highly relevant because it modulates the activity of antifolate chemotherapeutics such as methotrexate and pemetrexed. These drugs require polyglutamation by FPGS for retention and enhanced potency. Research using FPGS antibody has demonstrated that low expression of Folylpolyglutamate synthase confers resistance to antifolate therapy, while high activity sensitizes cells to treatment. This makes FPGS an important biomarker for predicting drug response in leukemia, lymphoma, and solid tumors. Studies continue to evaluate whether modulation of FPGS expression can overcome resistance and improve outcomes in antifolate-based regimens.\u003cbr\u003e\u003cbr\u003eBeyond cancer, folate metabolism plays critical roles in pregnancy, neural tube development, and cardiovascular health. Research with FPGS antibody has shown that adequate polyglutamate production is required for normal development, and deficiencies in this pathway contribute to congenital malformations and disease. As such, FPGS remains an important protein in both clinical and nutritional studies. Immunohistochemistry and western blotting using FPGS antibody have confirmed its widespread expression across tissues, with particular enrichment in proliferative and metabolically active cells.\u003cbr\u003e\u003cbr\u003eFPGS antibody is used in western blotting, immunohistochemistry, and enzyme activity assays. Western blotting measures relative abundance in normal and tumor tissue, immunohistochemistry demonstrates tissue distribution, and enzymatic assays link FPGS expression with catalytic activity. Together, these methods provide a full picture of how Folylpolyglutamate synthase supports cell metabolism, proliferation, and therapeutic responses.\u003cbr\u003e\u003cbr\u003eBy providing validated FPGS antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=FPGS - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FPGS - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=FPGS - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/FPGS - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=FPGS+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047308747117,"sku":"FY13003","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_447ae574-a8aa-4703-b0c4-81b7a40070f8.jpg?v=1772019374","url":"https:\/\/www.ebiohippo.com\/products\/fpgs-antibody-folylpolyglutamate-synthase-bha17135905","provider":"BioHippo","version":"1.0","type":"link"}