{"product_id":"frg1-antibody-fshd-region-gene-1-bha17136002","title":"FRG1 Antibody \/ FSHD region gene 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eFRG1 Antibody \/ FSHD region gene 1 is a anti-FRG1 Rabbit antibody Recombinant Rabbit Monoclonal clone 32F07 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF) with listed reactivity in Human, Mouse, Rat.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e FRG1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Recombinant Rabbit Monoclonal, clone 32F07, isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Liquid\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, ICC, IF\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eFRG1 antibody detects FSHD region gene 1 protein, encoded by the FRG1 gene. This protein is associated with RNA processing, pre-mRNA splicing, and actin cytoskeleton organization. FRG1 antibody provides researchers with a critical reagent for investigating muscular dystrophy, RNA metabolism, and nuclear architecture. Interest in FRG1 intensified because of its location within the genomic region associated with facioscapulohumeral muscular dystrophy (FSHD), a progressive muscle wasting disease.\u003cbr\u003e\u003cbr\u003eFSHD region gene 1 protein is expressed in both nuclear and cytoplasmic compartments. In the nucleus, it associates with splicing machinery and contributes to RNA maturation. Research using FRG1 antibody has demonstrated that overexpression of FRG1 alters splicing of muscle-specific transcripts, contributing to muscle pathology. In the cytoplasm, FRG1 interacts with actin filaments, influencing cytoskeletal organization and cell motility. These dual roles highlight its broad impact on cellular function.\u003cbr\u003e\u003cbr\u003eOverexpression of FRG1 has been shown to produce muscular dystrophy-like phenotypes in model organisms. Studies with FRG1 antibody have confirmed that excessive expression disrupts muscle development, leading to muscle weakness and degeneration. This evidence supports the hypothesis that misregulation of FRG1 contributes to FSHD pathology. Conversely, controlled levels of FRG1 appear necessary for normal muscle and vascular development, linking its regulation to tissue homeostasis.\u003cbr\u003e\u003cbr\u003eBeyond muscular dystrophy, FRG1 has been implicated in angiogenesis and vascular biology. Research with FRG1 antibody has revealed that it influences endothelial cell migration and capillary formation. Dysregulation of FRG1 may therefore contribute to vascular defects in addition to muscle abnormalities. This broadens the biological significance of FSHD region gene 1 protein to multiple tissues and disease processes.\u003cbr\u003e\u003cbr\u003eFRG1 antibody is widely used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting identifies both endogenous and overexpressed FRG1, immunohistochemistry localizes it in muscle tissue, and immunofluorescence shows colocalization with nuclear speckles and actin filaments. These applications make FRG1 antibody indispensable for muscular dystrophy research.\u003cbr\u003e\u003cbr\u003eBy supplying validated FRG1 antibody reagents,\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunofluorescence:\u003c\/strong\u003e visualize subcellular distribution and cell-to-cell heterogeneity.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=FRG1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FRG1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=FRG1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/FRG1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=FRG1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg\/ml BSA \/ 100 ul","offer_id":53047312843117,"sku":"FY13100","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_009b6ce6-abdc-4ca4-9901-98629cae12e8.jpg?v=1772019393","url":"https:\/\/www.ebiohippo.com\/products\/frg1-antibody-fshd-region-gene-1-bha17136002","provider":"BioHippo","version":"1.0","type":"link"}