{"product_id":"fundc1-antibody-fun14-domain-containing-protein-1-bha17135748","title":"FUNDC1 Antibody \/ FUN14 domain-containing protein 1","description":"\u003ch2\u003eOverview\u003c\/h2\u003e\u003cp\u003eFUNDC1 Antibody \/ FUN14 domain-containing protein 1 is a anti-FUNDC1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm.\u003c\/p\u003e\u003ch2\u003eKey elements and design rationale\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eTarget:\u003c\/strong\u003e FUNDC1\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eAntibody details:\u003c\/strong\u003e Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFormat:\u003c\/strong\u003e Lyophilized\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eApplications (as listed):\u003c\/strong\u003e WB, IHC, FACS\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eBiological background\u003c\/h2\u003e\u003cdiv\u003eFUNDC1 antibody detects FUN14 domain-containing protein 1, a mitochondrial outer membrane protein that regulates mitophagy and mitochondrial quality control. Encoded by the FUNDC1 gene on chromosome Xq26.1, this protein serves as a receptor for hypoxia-induced mitophagy, linking damaged or depolarized mitochondria to the autophagic machinery. FUNDC1 interacts with LC3 through its LC3-interacting region (LIR) motif, facilitating selective removal of dysfunctional mitochondria during cellular stress.\u003cbr\u003e\u003cbr\u003eStructurally, FUNDC1 contains three transmembrane helices that anchor it to the mitochondrial outer membrane, with its N-terminal domain exposed to the cytosol for binding autophagy-related proteins. Under normoxic conditions, FUNDC1 is phosphorylated at Tyr18 and Ser13, suppressing its interaction with LC3. During hypoxia or mitochondrial depolarization, phosphatases such as PGAM5 dephosphorylate these residues, enhancing its affinity for LC3 and triggering mitophagy. This dynamic post-translational regulation ensures precise control of mitochondrial turnover.\u003cbr\u003e\u003cbr\u003eThe FUNDC1 antibody is widely used in mitochondrial biology, autophagy, and metabolic research to study mitophagy signaling, organelle homeostasis, and cellular stress adaptation. Western blot analysis detects a 16 kilodalton band corresponding to FUNDC1, while immunofluorescence shows punctate staining along mitochondrial networks. This antibody enables researchers to monitor mitochondrial dynamics and degradation under conditions such as hypoxia, oxidative stress, or metabolic reprogramming.\u003cbr\u003e\u003cbr\u003eFUNDC1-mediated mitophagy is essential for maintaining energy balance and preventing accumulation of damaged mitochondria that could release pro-apoptotic or inflammatory signals. Dysregulation of FUNDC1 contributes to diseases such as cardiac ischemia, neurodegeneration, and cancer, where impaired mitophagy affects cell survival and metabolic fitness. The FUNDC1 antibody provides a sensitive reagent for assessing mitophagic flux and mitochondrial remodeling.\u003c\/div\u003e\u003ch2\u003eResearch relevance and current trends\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eConnecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).\u003c\/li\u003e\n\u003cli\u003eConsidering isoforms and post-translational regulation when interpreting protein-level changes.\u003c\/li\u003e\n\u003cli\u003eComparing results across species and model systems with matched controls.\u003c\/li\u003e\n\u003c\/ul\u003e\u003ch2\u003eCommon research applications\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003e\n\u003cstrong\u003eWestern blotting:\u003c\/strong\u003e compare relative abundance and activation-state changes across conditions.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eImmunohistochemistry:\u003c\/strong\u003e map target signal in tissue context and compare regions\/phenotypes.\u003c\/li\u003e\n\u003cli\u003e\n\u003cstrong\u003eFlow cytometry:\u003c\/strong\u003e quantify target-positive populations and signal shifts at single-cell resolution.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003eInterpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.\u003c\/p\u003e\u003ch2\u003eNotes for experimental interpretation\u003c\/h2\u003e\u003cul\u003e\n\u003cli\u003eSignal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.\u003c\/li\u003e\n\u003cli\u003eSpecies differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.\u003c\/li\u003e\n\u003c\/ul\u003e\u003cp\u003e\u003cstrong\u003eAntibody notes:\u003c\/strong\u003e Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.\u003c\/p\u003e\u003c!-- Sources (internal): - UniProt search — UniProt — https:\/\/www.uniprot.org\/uniprotkb?query=FUNDC1 - NCBI Gene search — NCBI — https:\/\/www.ncbi.nlm.nih.gov\/gene\/?term=FUNDC1 - Ensembl search — Ensembl — https:\/\/www.ensembl.org\/Multi\/Search\/Results?q=FUNDC1 - Human Protein Atlas search — HPA — https:\/\/www.proteinatlas.org\/search\/FUNDC1 - PubMed (review) — NLM — https:\/\/pubmed.ncbi.nlm.nih.gov\/?term=FUNDC1+review --\u003e","brand":"NSJ Bioreagents","offers":[{"title":"Adding 0.2 ml of distilled water will yield a concentration of 500 ug\/ml \/ 100 ug","offer_id":53047303700845,"sku":"FY12846","price":449.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/get_image_552586a5-0468-4fb6-a1a5-8026ea4823a7.jpg?v=1782237053","url":"https:\/\/www.ebiohippo.com\/products\/fundc1-antibody-fun14-domain-containing-protein-1-bha17135748","provider":"BioHippo","version":"1.0","type":"link"}