{"product_id":"fxre-er2-reporter-lentivirus-bhv19400237","title":"FXRE\/ER2 Reporter Lentivirus","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe farnesoid X receptor (FXR) is a bile acid-activated nuclear receptor that functions as a master regulator of metabolic homeostasis. Activated FXR heterodimerizes with the retinoid X receptor and binds FXR response elements (FXRE) to regulate genes controlling bile acid synthesis and transport, lipid and glucose metabolism, and amino acid handling. The FXRE arranged as an everted repeat separated by two nucleotides (ER2) preferentially responds to the FXR-alpha2 and FXR-alpha4 isoforms, allowing isoform-selective monitoring of FXR activity. FXR signaling protects against bile acid toxicity and helps maintain liver and intestinal health, and its dysregulation is linked to cholestasis, metabolic disease, and liver disorders, making it an important target in metabolism and signaling research.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThe FXRE\/ER2 Reporter Lentivirus is a transcription factor reporter system that detects transcriptional activity mediated by the farnesoid X receptor, with preferential readout of the FXR-alpha2 and FXR-alpha4 isoforms. The construct contains tandem FXR response elements arranged in the everted repeat ER2 configuration, placed upstream of a minimal promoter driving a fluorescent or luminescent reporter, with an optimized upstream enhancer that maximizes signal-to-noise. A constitutive drug selection marker (Blasticidin, Hygromycin, Puromycin, or Zeocin) enables generation of stable polyclonal reporter cell lines. Stable lentiviral integration provides consistent reporter expression suitable for fluorescence microscopy, flow cytometry, or luminometry. Supplied as high-titer particles purified by PEG precipitation and sucrose gradient centrifugation, it is suited to primary and difficult-to-transfect cells.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis reporter lentivirus places a BFP2, d2GFP, EGFP, Firefly Luc, Gaussia Luc, GFP, GFP + Firefly Luc, mCherry, Renilla Luc, RFP, RFP + Firefly Luc reporter gene under the control of tandem consensus response elements specific for the FXR\/ERa2 Pathway transcription factor, coupled to a minimal TATA-box promoter and a proprietary upstream enhancer that maximizes signal-to-noise. The constitutively expressed selection marker (Blasticidin, Hygromycin, Puromycin, Zeocin) and\/or secondary reporter enables stable polyclonal cell line generation and flexible readout by fluorescence microscopy, flow cytometry, or luminometry.\u003c\/p\u003e\n\u003cp\u003eStable integration via the lentiviral backbone ensures consistent, clonally representative reporter expression in dividing and post-mitotic target cells — including primary T cells, macrophages, organoids, and cryopreserved material — eliminating the variability inherent to transient transfection. The self-inactivating LTR design and third-generation packaging minimize insertional mutagenesis risk and ensure biosafety classification at BSL-2.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"FXRE\/ER2-TAG-Puro \/ GFP \/ 5x10^6","offer_id":53251616178541,"sku":"LTV-0130-1S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362089066861,"sku":"LTV-0130-1N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAG-Puro) \/ GFP \/ 5x10^6","offer_id":53362089099629,"sku":"LTV-0130-1P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FXRE\/ER2-TAL-Puro \/ Firefly Luc \/ 2x10^6","offer_id":53362089132397,"sku":"LTV-0130-3S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Negative Control (NC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362089165165,"sku":"LTV-0130-3N","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Positive Control (PC-TAL-Puro) \/ Firefly Luc \/ 2x10^6","offer_id":53362089197933,"sku":"LTV-0130-3P","price":595.0,"currency_code":"USD","in_stock":true},{"title":"Internal Control (RLuc-BSD) \/ Renilla Luc \/ 2x10^6","offer_id":53362089230701,"sku":"LTV-0130-3R","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FXRE\/ER2-TAR-BSD \/ RFP \/ 5x10^6","offer_id":53362089263469,"sku":"LTV-0130-2S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FXRE\/ER2-TAL-BSD \/ Firefly Luc \/ 2x10^6","offer_id":53362089296237,"sku":"LTV-0130-4S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FXRE\/ER2-TAG-BSD \/ GFP \/ 5x10^6","offer_id":53362089329005,"sku":"LTV-0130-5S","price":595.0,"currency_code":"USD","in_stock":true},{"title":"FXRE\/ER2-TAR-Puro \/ RFP \/ 5x10^6","offer_id":53362089361773,"sku":"LTV-0130-6S","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/fxre-er2-1.jpg?v=1776934151","url":"https:\/\/www.ebiohippo.com\/products\/fxre-er2-reporter-lentivirus-bhv19400237","provider":"BioHippo","version":"1.0","type":"link"}