{"product_id":"gal4-dbd-er-alpha-lbd-bsd-bhv19400186","title":"GAL4-DBD-ERα-LBD-BSD","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eEstrogen Receptor alpha (ERα, NR3A1), encoded by the ESR1 gene, is a ligand-activated nuclear receptor and one of the two classical intracellular estrogen receptors alongside ERβ. Upon binding estrogens such as 17β-estradiol, ERα translocates to the nucleus and regulates transcription of genes controlling growth, metabolism, and reproductive physiology. ERα signaling is a major driver of hormone-responsive breast cancer and a central target of endocrine therapies, making it important in both fundamental endocrinology and clinical oncology research. ERα is therefore widely studied to characterize ligand interactions and the pharmacology of selective estrogen receptor modulators.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis GAL4-DBD-ERα-LBD-BSD lentivirus expresses a chimeric protein in which the yeast GAL4 DNA-binding domain is fused to the ligand-binding domain (LBD) of human ERα. Upon ligand binding, the LBD changes conformation and recruits co-activators, and the chimera binds upstream GAL4 UAS elements to drive a fluorescent or luminescent reporter (GFP, RFP, or firefly luciferase). The GAL4-LBD design isolates LBD-mediated transactivation from endogenous nuclear receptor activity, giving high signal-to-noise and specificity for studying ligand interactions with ERα. The system is used to characterize ERα agonists, antagonists, and selective modulators in pharmacology and drug discovery, and is amenable to high-throughput screening by luminometry. Supplied as high-titer lentiviral particles that allow researchers to establish their own stable reporter cell lines, reducing repeated transfections.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis lentiviral system expresses a chimeric protein consisting of the GAL4 DNA-binding domain fused to the ligand-binding domain (LBD) of \u003cstrong\u003eERa\u003c\/strong\u003e. Upon specific ligand binding, the LBD undergoes conformational change and recruits co-activators; the activated chimera binds upstream GAL4 response elements (UAS×5) to drive expression of a fluorescent or luminescent reporter (GFP, RFP, or Firefly Luciferase) with high signal-to-noise relative to endogenous nuclear receptor background.\u003c\/p\u003e\n\u003cp\u003eThe GAL4-LBD design isolates LBD-mediated transactivation from A\/B domain-driven constitutive activity and prevents cross-reactivity with endogenous nuclear receptor targets, enabling highly specific pharmacological characterization of LBD agonists, antagonists, and selective receptor modulators (SARMs\/SERMs\/SPPARMs). This system is established for drug discovery in academic and industrial settings, and is amenable to high-throughput screening (HTS) formats using luminometry.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GAL4-DBD-ERα-LBD-BSD \/ Blasticidin \/ 3x10^6","offer_id":53251619750253,"sku":"GNV-0002-1S","price":995.0,"currency_code":"USD","in_stock":true},{"title":"GAL4-TAG-Puro \/ Puromycin \/ 5x10^6","offer_id":53362031133037,"sku":"GNV-0002-1G","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GAL4-TAR-Puro \/ Puromycin \/ 5x10^6","offer_id":53362031165805,"sku":"GNV-0002-6G","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GAL4-TAL-Puro \/ Puromycin \/ 2x10^6","offer_id":53362031198573,"sku":"GNV-0002-3G","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/GAL4-ERa-Thumbnail.jpg?v=1782157779","url":"https:\/\/www.ebiohippo.com\/products\/gal4-dbd-er-alpha-lbd-bsd-bhv19400186","provider":"BioHippo","version":"1.0","type":"link"}