{"product_id":"gal4-dbd-nr1d1-lbd-vp16-bsd-bhv19400192","title":"GAL4-DBD-NR1D1-LBD-VP16-BSD","description":"\u003cdiv class=\"bhp-desc\"\u003e\n\u003cstyle\u003e.bhp-desc{font-size:16px;color:#1a1a1a;line-height:1.7}.bhp-desc h2{font-size:18px;font-weight:700;color:#003366;margin:24px 0 10px;padding-bottom:6px;border-bottom:2px solid #003366}.bhp-desc p{margin:0 0 12px}.bhp-desc ul{margin:0 0 12px 22px}.bhp-desc li{margin:0 0 6px}\u003c\/style\u003e\n\u003ch2\u003e\u003cstrong\u003eBackground\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eNR1D1 encodes REV-ERBα, a nuclear receptor that acts as a transcriptional repressor and a core component of the circadian clock. REV-ERBα itself is expressed in a circadian pattern, particularly in metabolic tissues such as liver, skeletal muscle, adipose tissue, and brain, and it represses positive clock genes to maintain rhythmic timing. Through its target genes, REV-ERBα links the molecular clock to lipid metabolism, glucose homeostasis, and inflammatory responses, helping coordinate physiology with environmental cues. Because of these roles, REV-ERBα and its ligand-binding domain are important targets in circadian biology, metabolic disease research, and drug discovery.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eProduct Description \u0026amp; Applications\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis GAL4-nuclear-receptor reporter lentivirus expresses a chimeric protein in which the GAL4 DNA-binding domain is fused to the ligand-binding domain (LBD) of NR1D1 (REV-ERBα) together with a VP16 activation domain, with blasticidin selection available. Ligand binding to the LBD drives the chimera to upstream GAL4 (UAS) response elements, activating a fluorescent or luminescent reporter such as GFP, RFP, or firefly luciferase with high signal-to-noise relative to endogenous nuclear receptor background.\u003c\/p\u003e\n\u003cp\u003eBy isolating LBD-mediated transactivation from endogenous receptor targets, the system enables specific pharmacological characterization of REV-ERBα ligands, including agonists and antagonists. Supplied as high-titer lentiviral particles, it supports establishment of stable cell lines for reproducible results, is amenable to high-throughput screening by luminometry, and avoids repeated transfections in circadian and metabolic drug discovery.\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003eAbout This Product\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp\u003eThis lentiviral system expresses a chimeric protein consisting of the GAL4 DNA-binding domain fused to the ligand-binding domain (LBD) of \u003cstrong\u003eNR1D1\u003c\/strong\u003e. Upon specific ligand binding, the LBD undergoes conformational change and recruits co-activators; the activated chimera binds upstream GAL4 response elements (UAS×5) to drive expression of a fluorescent or luminescent reporter (GFP, RFP, or Firefly Luciferase) with high signal-to-noise relative to endogenous nuclear receptor background.\u003c\/p\u003e\n\u003cp\u003eThe GAL4-LBD design isolates LBD-mediated transactivation from A\/B domain-driven constitutive activity and prevents cross-reactivity with endogenous nuclear receptor targets, enabling highly specific pharmacological characterization of LBD agonists, antagonists, and selective receptor modulators (SARMs\/SERMs\/SPPARMs). This system is established for drug discovery in academic and industrial settings, and is amenable to high-throughput screening (HTS) formats using luminometry.\u003c\/p\u003e\n\u003c\/div\u003e","brand":"LipExoGen Biotech","offers":[{"title":"GAL4-DBD-NR1D1-LBD-VP16-BSD \/ Blasticidin \/ 3x10^6","offer_id":53251622109549,"sku":"GNV-0008-1S","price":995.0,"currency_code":"USD","in_stock":true},{"title":"GAL4-TAG-Puro \/ Puromycin \/ 5x10^6","offer_id":53362031919469,"sku":"GNV-0008-1G","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GAL4-TAR-Puro \/ Puromycin \/ 5x10^6","offer_id":53362031952237,"sku":"GNV-0008-6G","price":595.0,"currency_code":"USD","in_stock":true},{"title":"GAL4-TAL-Puro \/ Puromycin \/ 2x10^6","offer_id":53362031985005,"sku":"GNV-0008-3G","price":595.0,"currency_code":"USD","in_stock":true}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0949\/7424\/7277\/files\/GAL4-NR1D1-Thumbnail.jpg?v=1782157779","url":"https:\/\/www.ebiohippo.com\/products\/gal4-dbd-nr1d1-lbd-vp16-bsd-bhv19400192","provider":"BioHippo","version":"1.0","type":"link"}